The effect of 6-mercaptopurine and its derivatives on mammalian RNA-dependent RNA synthesis

Kathleen M. Downey, John J. Byrnes, Bonnie S. Jurmark, Antero G. So

Research output: Contribution to journalArticle

2 Scopus citations

Abstract

The effect of 6-mercaptopurine (6-MP) and its nucleoside and nucleotide derivatives on the rate of RNA synthesis with reticulocyte RNA-dependent RNA polymerase has been examined. Of the 6-MP derivatives tested only 6-mercaptopurine ribo-5′-monophosphate (6-MPR-P) significantly stimulates the rate of RNA synthesis with hemoglobin mRNA as a template while 6-MP and 6-MPR have little or no effect. Kinetic studies have demonstrated that 6-MPR-P activates the RNA-dependent RNA polymerase by increasing the Vmax of the enzyme rather than by decreasing the Km values for the substrates. It is suggested that the stimulation of RNA-dependent RNA synthesis by 6-MPR-P may explain the transient development of megaloblastic anemia following 6-MP therapy.

Original languageEnglish (US)
Pages (from-to)227-233
Number of pages7
JournalBiochemical and biophysical research communications
Volume56
Issue number1
DOIs
StatePublished - Jan 7 1974

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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