The effect of 6-mercaptopurine and its derivatives on mammalian RNA-dependent RNA synthesis

Kathleen M. Downey, John Byrnes, Bonnie S. Jurmark, Antero G. So

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

The effect of 6-mercaptopurine (6-MP) and its nucleoside and nucleotide derivatives on the rate of RNA synthesis with reticulocyte RNA-dependent RNA polymerase has been examined. Of the 6-MP derivatives tested only 6-mercaptopurine ribo-5′-monophosphate (6-MPR-P) significantly stimulates the rate of RNA synthesis with hemoglobin mRNA as a template while 6-MP and 6-MPR have little or no effect. Kinetic studies have demonstrated that 6-MPR-P activates the RNA-dependent RNA polymerase by increasing the Vmax of the enzyme rather than by decreasing the Km values for the substrates. It is suggested that the stimulation of RNA-dependent RNA synthesis by 6-MPR-P may explain the transient development of megaloblastic anemia following 6-MP therapy.

Original languageEnglish
Pages (from-to)227-233
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume56
Issue number1
DOIs
StatePublished - Jan 7 1974

Fingerprint

6-Mercaptopurine
RNA
Derivatives
RNA Replicase
Megaloblastic Anemia
Reticulocytes
Nucleosides
Hemoglobins
Nucleotides
Messenger RNA
Kinetics
Substrates
Enzymes

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

The effect of 6-mercaptopurine and its derivatives on mammalian RNA-dependent RNA synthesis. / Downey, Kathleen M.; Byrnes, John; Jurmark, Bonnie S.; So, Antero G.

In: Biochemical and Biophysical Research Communications, Vol. 56, No. 1, 07.01.1974, p. 227-233.

Research output: Contribution to journalArticle

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