The early kinetics of gentamicin uptake into the inner ear

Michael E. Hoffer, Keith Allen, Kim Gottshall, Robert Moore, Richard D. Kopke, Derin Wester, Carey Balaban

Research output: Contribution to journalArticle

2 Scopus citations

Abstract

Transtympanic gentamicin administration has become a popular modality in the treatment of Ménière's disease. This modality and other inner-ear medical therapy are gaining increased clinical and scientific attention. We previously described the kinetics and effects of gentamicin uptake into the inner ear after delivery of the medicine into the middle ear using a variety of different techniques and sustained-release modalities [1]. In our previous work, we reported an early peak perilymph concentration and the presence of intracellular gentamicin at the 4-hour time point. We also demonstrated the activation of inner-ear damage pathways at this early time point. In this report, we examine the kinetics of gentamicin at very early time points, 1 and 2 hours after administration. Healthy adult chinchillas underwent implantation of middle-ear sustained-release devices (one to each ear) containing gentamicin. The animals then were maintained in a neutral position and underwent perilymph gentamicin sampling at the two predetermined time points. This technique allowed us to assess accurately very early time point inner-ear gentamicin kinetics and to compare the activity. The samples then were run for concentration using mass spectrometry. The information gained from this study may increase our scientific understanding about the effects of gentamicin on the inner ear and may allow clinicians to treat patients more effectively for inner-ear disorders.

Original languageEnglish (US)
Pages (from-to)27-29
Number of pages3
JournalInternational Tinnitus Journal
Volume8
Issue number1
StatePublished - Jul 9 2002
Externally publishedYes

Keywords

  • Fluorescent polar immunoassay
  • Gentamicin
  • Mass spectrometry
  • Ménière's disease
  • Sustained-release

ASJC Scopus subject areas

  • Otorhinolaryngology
  • Sensory Systems
  • Speech and Hearing

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  • Cite this

    Hoffer, M. E., Allen, K., Gottshall, K., Moore, R., Kopke, R. D., Wester, D., & Balaban, C. (2002). The early kinetics of gentamicin uptake into the inner ear. International Tinnitus Journal, 8(1), 27-29.