The dynamics of SAP90/PSD-95 recruitment to new synaptic junctions

Tal Bresler, Yaron Ramati, Pedro L. Zamorano, Rong G Zhai, Craig C. Garner, Noam E. Ziv

Research output: Contribution to journalArticle

88 Citations (Scopus)

Abstract

SAP90/PSD-95 is thought to be a central organizer of the glutamatergic synapse postsynaptic reception apparatus. To assess its potential role during glutamatergic synapse formation, we used GFP-tagged SAP90/PSD-95, time lapse confocal microscopy, and cultured hippocampal neurons to determine its dynamic recruitment into new synaptic junctions. We report that new SAP90/PSD-95 clusters first appeared at new axodendritic contact sites within 20-60 min of contact establishment. SAP90/PSD-95 clustering was rapid, with kinetics that fit a single exponential with a mean time constant of ∼23 min. Most new SAP90/PSD-95 clusters were found juxtaposed to functional presynaptic boutons as determined by labeling with FM 4-64. No evidence was found for the existence of discrete transport particles similar to those previously reported to mediate presynaptic active zone cytoskeleton assembly. Instead, we found that SAP90/PSD-95 is recruited to nascent synapses from a diffuse dendritic cytoplasmic pool. Our findings show that SAP90/PSD-95 is recruited to nascent synaptic junctions early during the assembly process and indicate that its assimilation is fundamentally different from that of presynaptic active zone components.

Original languageEnglish
Pages (from-to)149-167
Number of pages19
JournalMolecular and Cellular Neuroscience
Volume18
Issue number2
DOIs
StatePublished - Sep 26 2001
Externally publishedYes

Fingerprint

Synapses
Cytoskeleton
Confocal Microscopy
Cluster Analysis
Neurons
FM 4-64

ASJC Scopus subject areas

  • Molecular Biology
  • Cellular and Molecular Neuroscience
  • Developmental Neuroscience

Cite this

The dynamics of SAP90/PSD-95 recruitment to new synaptic junctions. / Bresler, Tal; Ramati, Yaron; Zamorano, Pedro L.; Zhai, Rong G; Garner, Craig C.; Ziv, Noam E.

In: Molecular and Cellular Neuroscience, Vol. 18, No. 2, 26.09.2001, p. 149-167.

Research output: Contribution to journalArticle

Bresler, Tal ; Ramati, Yaron ; Zamorano, Pedro L. ; Zhai, Rong G ; Garner, Craig C. ; Ziv, Noam E. / The dynamics of SAP90/PSD-95 recruitment to new synaptic junctions. In: Molecular and Cellular Neuroscience. 2001 ; Vol. 18, No. 2. pp. 149-167.
@article{fc811d517c17479b948b0b6a72a060e3,
title = "The dynamics of SAP90/PSD-95 recruitment to new synaptic junctions",
abstract = "SAP90/PSD-95 is thought to be a central organizer of the glutamatergic synapse postsynaptic reception apparatus. To assess its potential role during glutamatergic synapse formation, we used GFP-tagged SAP90/PSD-95, time lapse confocal microscopy, and cultured hippocampal neurons to determine its dynamic recruitment into new synaptic junctions. We report that new SAP90/PSD-95 clusters first appeared at new axodendritic contact sites within 20-60 min of contact establishment. SAP90/PSD-95 clustering was rapid, with kinetics that fit a single exponential with a mean time constant of ∼23 min. Most new SAP90/PSD-95 clusters were found juxtaposed to functional presynaptic boutons as determined by labeling with FM 4-64. No evidence was found for the existence of discrete transport particles similar to those previously reported to mediate presynaptic active zone cytoskeleton assembly. Instead, we found that SAP90/PSD-95 is recruited to nascent synapses from a diffuse dendritic cytoplasmic pool. Our findings show that SAP90/PSD-95 is recruited to nascent synaptic junctions early during the assembly process and indicate that its assimilation is fundamentally different from that of presynaptic active zone components.",
author = "Tal Bresler and Yaron Ramati and Zamorano, {Pedro L.} and Zhai, {Rong G} and Garner, {Craig C.} and Ziv, {Noam E.}",
year = "2001",
month = "9",
day = "26",
doi = "10.1006/mcne.2001.1012",
language = "English",
volume = "18",
pages = "149--167",
journal = "Molecular and Cellular Neuroscience",
issn = "1044-7431",
publisher = "Academic Press Inc.",
number = "2",

}

TY - JOUR

T1 - The dynamics of SAP90/PSD-95 recruitment to new synaptic junctions

AU - Bresler, Tal

AU - Ramati, Yaron

AU - Zamorano, Pedro L.

AU - Zhai, Rong G

AU - Garner, Craig C.

AU - Ziv, Noam E.

PY - 2001/9/26

Y1 - 2001/9/26

N2 - SAP90/PSD-95 is thought to be a central organizer of the glutamatergic synapse postsynaptic reception apparatus. To assess its potential role during glutamatergic synapse formation, we used GFP-tagged SAP90/PSD-95, time lapse confocal microscopy, and cultured hippocampal neurons to determine its dynamic recruitment into new synaptic junctions. We report that new SAP90/PSD-95 clusters first appeared at new axodendritic contact sites within 20-60 min of contact establishment. SAP90/PSD-95 clustering was rapid, with kinetics that fit a single exponential with a mean time constant of ∼23 min. Most new SAP90/PSD-95 clusters were found juxtaposed to functional presynaptic boutons as determined by labeling with FM 4-64. No evidence was found for the existence of discrete transport particles similar to those previously reported to mediate presynaptic active zone cytoskeleton assembly. Instead, we found that SAP90/PSD-95 is recruited to nascent synapses from a diffuse dendritic cytoplasmic pool. Our findings show that SAP90/PSD-95 is recruited to nascent synaptic junctions early during the assembly process and indicate that its assimilation is fundamentally different from that of presynaptic active zone components.

AB - SAP90/PSD-95 is thought to be a central organizer of the glutamatergic synapse postsynaptic reception apparatus. To assess its potential role during glutamatergic synapse formation, we used GFP-tagged SAP90/PSD-95, time lapse confocal microscopy, and cultured hippocampal neurons to determine its dynamic recruitment into new synaptic junctions. We report that new SAP90/PSD-95 clusters first appeared at new axodendritic contact sites within 20-60 min of contact establishment. SAP90/PSD-95 clustering was rapid, with kinetics that fit a single exponential with a mean time constant of ∼23 min. Most new SAP90/PSD-95 clusters were found juxtaposed to functional presynaptic boutons as determined by labeling with FM 4-64. No evidence was found for the existence of discrete transport particles similar to those previously reported to mediate presynaptic active zone cytoskeleton assembly. Instead, we found that SAP90/PSD-95 is recruited to nascent synapses from a diffuse dendritic cytoplasmic pool. Our findings show that SAP90/PSD-95 is recruited to nascent synaptic junctions early during the assembly process and indicate that its assimilation is fundamentally different from that of presynaptic active zone components.

UR - http://www.scopus.com/inward/record.url?scp=0034857822&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0034857822&partnerID=8YFLogxK

U2 - 10.1006/mcne.2001.1012

DO - 10.1006/mcne.2001.1012

M3 - Article

VL - 18

SP - 149

EP - 167

JO - Molecular and Cellular Neuroscience

JF - Molecular and Cellular Neuroscience

SN - 1044-7431

IS - 2

ER -