Tritiated LH-RH was prepared by the Wilzbach method and repurified. After the injection of 1.0μCi of tritiated LH-RH into normal female rats, radioactivity disappeared rapidly from the circulation. The half-life of tritiated LH-RH, calculated from the disappearance curves, was about 7 min. Tritiated LH-RH was distributed in a volume of 38.4 ml or about 16% of the body weight. After 60 min, approximately 15% of the radioactivity was found in the urine. A significant accumulation of radioactivity, indicated by tissue to plasma ratio (T/P) greater than 1.0, occurred in the pineal gland, anterior and posterior pituitaries, the liver and kidney, but not in the cerebral cortex, hypothalamus, muscle tissue or adrenal glands. Concentration of the radioactivity by the liver and kidney indicates that these tissues are major sites for the inactivation and excretion of LH-RH.
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
- Pharmacology, Toxicology and Pharmaceutics(all)