The disappearance, excretion, and metabolism of tritiated prolyl leucyl glycinamide in man

T. W. Redding, A. J. Kastin, D. Gonzalez-Barcena, D. H. Coy, Y. Hirotsu, J. Ruelas, Andrew V Schally

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

3H Pro Leu Gly NH 2, a melanocyte stimulating hormone release inhibiting factor (MIF), was injected into 5 healthy men. A plot of the time of the disappearance of radioactivity in plasma was characteristic of a multiexponential curve. The half life of the first component was 1.9±0.06 min and of the second component 15.2±1.5 min. The distribution volume was 20% of the body weight. One hour after the injection of labeled MIF, 17.2±0.7% of the dose of radioactivity was found in the urine and after 24 hr 25% had been excreted. Extraction of the urine, followed by chromatography on carboxymethyl cellulose and thin layer electrophoresis, showed that over 70% of the radioactivity was associated with the intact molecule while a smaller amount appeared to be either free proline, Pro Leu OH, or the deaminated form of the tripeptide. It appears that the main pathway for the clearance of MIF from the human body is excretion of the intact molecule.

Original languageEnglish
Pages (from-to)119-126
Number of pages8
JournalNeuroendocrinology
Volume16
Issue number2
StatePublished - Dec 1 1974
Externally publishedYes

Fingerprint

MSH Release-Inhibiting Hormone
Radioactivity
Urine
Carboxymethylcellulose Sodium
Human Body
Proline
Half-Life
Electrophoresis
Chromatography
Body Weight
Injections

ASJC Scopus subject areas

  • Endocrinology
  • Neuroscience(all)

Cite this

Redding, T. W., Kastin, A. J., Gonzalez-Barcena, D., Coy, D. H., Hirotsu, Y., Ruelas, J., & Schally, A. V. (1974). The disappearance, excretion, and metabolism of tritiated prolyl leucyl glycinamide in man. Neuroendocrinology, 16(2), 119-126.

The disappearance, excretion, and metabolism of tritiated prolyl leucyl glycinamide in man. / Redding, T. W.; Kastin, A. J.; Gonzalez-Barcena, D.; Coy, D. H.; Hirotsu, Y.; Ruelas, J.; Schally, Andrew V.

In: Neuroendocrinology, Vol. 16, No. 2, 01.12.1974, p. 119-126.

Research output: Contribution to journalArticle

Redding, TW, Kastin, AJ, Gonzalez-Barcena, D, Coy, DH, Hirotsu, Y, Ruelas, J & Schally, AV 1974, 'The disappearance, excretion, and metabolism of tritiated prolyl leucyl glycinamide in man', Neuroendocrinology, vol. 16, no. 2, pp. 119-126.
Redding TW, Kastin AJ, Gonzalez-Barcena D, Coy DH, Hirotsu Y, Ruelas J et al. The disappearance, excretion, and metabolism of tritiated prolyl leucyl glycinamide in man. Neuroendocrinology. 1974 Dec 1;16(2):119-126.
Redding, T. W. ; Kastin, A. J. ; Gonzalez-Barcena, D. ; Coy, D. H. ; Hirotsu, Y. ; Ruelas, J. ; Schally, Andrew V. / The disappearance, excretion, and metabolism of tritiated prolyl leucyl glycinamide in man. In: Neuroendocrinology. 1974 ; Vol. 16, No. 2. pp. 119-126.
@article{66d153f030d44fd18fb850609ff8d8d1,
title = "The disappearance, excretion, and metabolism of tritiated prolyl leucyl glycinamide in man",
abstract = "3H Pro Leu Gly NH 2, a melanocyte stimulating hormone release inhibiting factor (MIF), was injected into 5 healthy men. A plot of the time of the disappearance of radioactivity in plasma was characteristic of a multiexponential curve. The half life of the first component was 1.9±0.06 min and of the second component 15.2±1.5 min. The distribution volume was 20{\%} of the body weight. One hour after the injection of labeled MIF, 17.2±0.7{\%} of the dose of radioactivity was found in the urine and after 24 hr 25{\%} had been excreted. Extraction of the urine, followed by chromatography on carboxymethyl cellulose and thin layer electrophoresis, showed that over 70{\%} of the radioactivity was associated with the intact molecule while a smaller amount appeared to be either free proline, Pro Leu OH, or the deaminated form of the tripeptide. It appears that the main pathway for the clearance of MIF from the human body is excretion of the intact molecule.",
author = "Redding, {T. W.} and Kastin, {A. J.} and D. Gonzalez-Barcena and Coy, {D. H.} and Y. Hirotsu and J. Ruelas and Schally, {Andrew V}",
year = "1974",
month = "12",
day = "1",
language = "English",
volume = "16",
pages = "119--126",
journal = "Neuroendocrinology",
issn = "0028-3835",
publisher = "S. Karger AG",
number = "2",

}

TY - JOUR

T1 - The disappearance, excretion, and metabolism of tritiated prolyl leucyl glycinamide in man

AU - Redding, T. W.

AU - Kastin, A. J.

AU - Gonzalez-Barcena, D.

AU - Coy, D. H.

AU - Hirotsu, Y.

AU - Ruelas, J.

AU - Schally, Andrew V

PY - 1974/12/1

Y1 - 1974/12/1

N2 - 3H Pro Leu Gly NH 2, a melanocyte stimulating hormone release inhibiting factor (MIF), was injected into 5 healthy men. A plot of the time of the disappearance of radioactivity in plasma was characteristic of a multiexponential curve. The half life of the first component was 1.9±0.06 min and of the second component 15.2±1.5 min. The distribution volume was 20% of the body weight. One hour after the injection of labeled MIF, 17.2±0.7% of the dose of radioactivity was found in the urine and after 24 hr 25% had been excreted. Extraction of the urine, followed by chromatography on carboxymethyl cellulose and thin layer electrophoresis, showed that over 70% of the radioactivity was associated with the intact molecule while a smaller amount appeared to be either free proline, Pro Leu OH, or the deaminated form of the tripeptide. It appears that the main pathway for the clearance of MIF from the human body is excretion of the intact molecule.

AB - 3H Pro Leu Gly NH 2, a melanocyte stimulating hormone release inhibiting factor (MIF), was injected into 5 healthy men. A plot of the time of the disappearance of radioactivity in plasma was characteristic of a multiexponential curve. The half life of the first component was 1.9±0.06 min and of the second component 15.2±1.5 min. The distribution volume was 20% of the body weight. One hour after the injection of labeled MIF, 17.2±0.7% of the dose of radioactivity was found in the urine and after 24 hr 25% had been excreted. Extraction of the urine, followed by chromatography on carboxymethyl cellulose and thin layer electrophoresis, showed that over 70% of the radioactivity was associated with the intact molecule while a smaller amount appeared to be either free proline, Pro Leu OH, or the deaminated form of the tripeptide. It appears that the main pathway for the clearance of MIF from the human body is excretion of the intact molecule.

UR - http://www.scopus.com/inward/record.url?scp=0016293921&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0016293921&partnerID=8YFLogxK

M3 - Article

VL - 16

SP - 119

EP - 126

JO - Neuroendocrinology

JF - Neuroendocrinology

SN - 0028-3835

IS - 2

ER -