The deubiquitinating protein USP24 interacts with DDB2 and regulates DDB2 stability

Ling Zhang, Abigail Lubin, Hua Chen, Zhongyi Sun, Feng Gong

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

Damage-specific DNA-binding protein 2 (DDB2) was first isolated as a subunit of the UV-DDB heterodimeric complex that is involved in DNA damage recognition in the nucleotide excision repair pathway (NER). DDB2 is required for efficient repair of CPDs in chromatin and is a component of the CRL4 DDB2 E3 ligase that targets XPC, histones and DDB2 itself for ubiquitination. In this study, a yeast two-hybrid screening of a human cDNA library was performed to identify potential DDB2 cellular partners. We identified a deubiquitinating enzyme, USP24, as a likely DDB2-interacting partner. Interaction between DDB2 and USP24 was confirmed by co-precipitation. Importantly, knockdown of USP24 in two human cell lines decreased the steady-state levels of DDB2, indicating that USP24-mediated DDB2 deubiquitination prevents DDB2 degradation. In addition, we demonstrated that USP24 can cleave an ubiquitinated form of DDB2 in vitro. Taken together, our results suggest that the ubiquitin-specific protease USP24 is a novel regulator of DDB2 stability.

Original languageEnglish
Pages (from-to)4378-4384
Number of pages7
JournalCell Cycle
Volume11
Issue number23
DOIs
StatePublished - Dec 1 2012

Fingerprint

Protein Stability
DNA-Binding Proteins
Proteins
Ubiquitin-Specific Proteases
Ubiquitin-Protein Ligases
Ubiquitination
Gene Library
DNA Repair
Histones
Proteolysis
DNA Damage
Chromatin
Yeasts

Keywords

  • DDB2
  • Deubiquitinase
  • Deubiquitination
  • Nucleotide excision repair
  • USP24
  • UV-DDB
  • XPE
  • Yeast two-hybrid

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology
  • Developmental Biology

Cite this

The deubiquitinating protein USP24 interacts with DDB2 and regulates DDB2 stability. / Zhang, Ling; Lubin, Abigail; Chen, Hua; Sun, Zhongyi; Gong, Feng.

In: Cell Cycle, Vol. 11, No. 23, 01.12.2012, p. 4378-4384.

Research output: Contribution to journalArticle

Zhang, Ling ; Lubin, Abigail ; Chen, Hua ; Sun, Zhongyi ; Gong, Feng. / The deubiquitinating protein USP24 interacts with DDB2 and regulates DDB2 stability. In: Cell Cycle. 2012 ; Vol. 11, No. 23. pp. 4378-4384.
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