The DEAD box protein Mrh4 functions in the assembly of the mitochondrial large ribosomal subunit

Dasmanthie De Silva, Flavia Fontanesi, Antonio Barrientos

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Proteins in a cell are universally synthesized by ribosomes. Mitochondria contain their own ribosomes, which specialize in the synthesis of a handful of proteins required for oxidative phosphorylation. The pathway of mitoribosomal biogenesis and factors involved are poorly characterized. An example is the DEAD box proteins, widely known to participate in the biogenesis of bacterial and cytoplasmic eukaryotic ribosomes as either RNA helicases or RNA chaperones, whose mitochondrial counterparts remain completely unknown. Here, we have identified the Saccharomyces cerevisiae mitochondrial DEAD box protein Mrh4 as essential for large mitoribosome subunit biogenesis. Mrh4 interacts with the 21S rRNA, mitoribosome subassemblies, and fully assembled mitoribosomes. In the absence of Mrh4, the 21S rRNA is matured and forms part of a large on-pathway assembly intermediate missing proteins Mrpl16 and Mrpl39. We conclude that Mrh4 plays an essential role during the late stages of mitoribosome assembly by promoting remodeling of the 21S rRNA-protein interactions.

Original languageEnglish
Pages (from-to)712-725
Number of pages14
JournalCell Metabolism
Volume18
Issue number5
DOIs
StatePublished - Nov 5 2013

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Large Ribosome Subunits
Ribosomes
Proteins
RNA Helicases
Oxidative Phosphorylation
Saccharomyces cerevisiae
Mitochondria
RNA
Mitochondrial Ribosomes

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology
  • Physiology

Cite this

The DEAD box protein Mrh4 functions in the assembly of the mitochondrial large ribosomal subunit. / De Silva, Dasmanthie; Fontanesi, Flavia; Barrientos, Antonio.

In: Cell Metabolism, Vol. 18, No. 5, 05.11.2013, p. 712-725.

Research output: Contribution to journalArticle

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