The cytokinetic basis for the design of efficacious radiotherapy protocols

Paul G Braunschweiger, Larry L. Schenken, Lewis M. Schiffer

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Studies were performed to investigate the perturbing effects of ionizing radiations on the cell kinetics of T1699 transplantable mouse mammary tumors (MMT). Cell kinetic parameters included the 3H-TdR labeling index (LI), the DNA synthesis time (TS) and the primer dependent DNA polymerase labeling index (PDPI), an estimate of tumor growth fraction; they were determined by in vitro methods at various times after acute (400-1200 R) and fractionated (1000 R) X-irradiation. The cell kinetic response after both acute and fractionated treatment was generally similar and could be divided into four phases. (1) The initial phase of the response, occurring within the first 24 hr, was characterized by a decrease in 3H-TdR LI, an increase in PDPI and a concomitant lengthening of the TS. (2) The second phase was characterized by a variable, dose dependent interval of decreased cellular proliferation, as evidenced by a lengthening of TS and decreases in both PDPI and 3H-TdR LI. (3) The third phase of the response, the recovery phase, was characterized by increased cellular proliferation as evidenced by the recovery of TS to normal and increases in both PDPI and 3H-TdR LI to above control levels, in most cases. (4) The fourth phase of the response was characterized by a reestablishment of normal proliferative patterns. Studies with continuing radiotherapy schedules showed that the most effective schedules for local control and ILS were those in which radiation fractions were given just prior to initiation of observed proliferative recovery. The least effective schedules were those in which radiation fractions coincided with the time of maximal proliferative activity.

Original languageEnglish
Pages (from-to)37-47
Number of pages11
JournalInternational journal of radiation oncology, biology, physics
Volume5
Issue number1
DOIs
StatePublished - Jan 1 1979
Externally publishedYes

Fingerprint

DNA-Directed DNA Polymerase
marking
radiation therapy
Radiotherapy
primers
Appointments and Schedules
deoxyribonucleic acid
schedules
Cell Proliferation
Radiation
recovery
Ionizing Radiation
instrument landing systems
kinetics
tumors
cells
Breast Neoplasms
DNA
radiation
Growth

Keywords

  • Cell kinetics
  • Mammary tumors
  • X-irradiation

ASJC Scopus subject areas

  • Oncology
  • Radiology Nuclear Medicine and imaging
  • Radiation

Cite this

The cytokinetic basis for the design of efficacious radiotherapy protocols. / Braunschweiger, Paul G; Schenken, Larry L.; Schiffer, Lewis M.

In: International journal of radiation oncology, biology, physics, Vol. 5, No. 1, 01.01.1979, p. 37-47.

Research output: Contribution to journalArticle

Braunschweiger, Paul G ; Schenken, Larry L. ; Schiffer, Lewis M. / The cytokinetic basis for the design of efficacious radiotherapy protocols. In: International journal of radiation oncology, biology, physics. 1979 ; Vol. 5, No. 1. pp. 37-47.
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