The cultured branchial epithelium of the rainbow trout as a model for diffusive fluxes of ammonia across the fish gill

Scott P. Kelly, Chris M. Wood

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

A novel branchial epithelial preparation grown in L-15 medium in culture was used as a model system for understanding the diffusion of ammonia across the gills of the rainbow trout Oncorhynchus mykiss. The epithelium is known to contain both respiratory and mitochondria-rich cells in the approximate proportion in which they occur in vivo and to exhibit diffusive fluxes of Na+ and Cl- similar to in vivo values, but does not exhibit active apical-to-basolateral transport of Na+. Transepithelial resistance and paracellular permeability are also known to increase when the apical medium is changed from L-15 medium (symmetrical conditions) to fresh water (asymmetrical conditions). In the present study, net basolateral-to-apical ammonia fluxes increased as basolateral total ammonia concentration, basolateral-to-apical pH gradients and basolateral-to-apical PNH3 gradients were experimentally increased and were greater under asymmetrical than under symmetrical conditions. The slope of the relationship between ammonia flux and PNH3 gradient (i.e. NH3 permeability) was the same under both conditions and similar to values for other epithelia. The higher fluxes under asymmetrical conditions were explained by an apparent diffusive flux of NH4+ that was linearly correlated with transepithelial conductance and was probably explained by the higher electrochemical gradient and higher paracellular permeability when fresh water was present on the apical surface. In this situation, NH4+ diffusion was greater than NH3 diffusion under conditions representative of in vivo values, but overall fluxes amounted to only approximately 20% of those in vivo. These results suggest that branchial ammonia excretion in the intact animal is unlikely to be explained by diffusion alone and, therefore, that carrier-mediated transport may play an important role.

Original languageEnglish
Pages (from-to)4115-4124
Number of pages10
JournalJournal of Experimental Biology
Volume204
Issue number23
StatePublished - Dec 1 2001

Fingerprint

Oncorhynchus mykiss
Ammonia
rainbow
gills
Fishes
ammonia
epithelium
Epithelium
fish
Permeability
permeability
Fresh Water
Proton-Motive Force
mitochondrion
excretion
Culture Media
Mitochondria
water
mitochondria
animal

Keywords

  • Ammonia diffusion
  • Cultured epithelium
  • Gill
  • NH electrochemical gradient
  • Oncorhynchus mykiss
  • P gradient
  • Paracellular pathway
  • pH gradient
  • Rainbow trout
  • Transepithelial conductance
  • Transepithelial resistance

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Agricultural and Biological Sciences (miscellaneous)

Cite this

The cultured branchial epithelium of the rainbow trout as a model for diffusive fluxes of ammonia across the fish gill. / Kelly, Scott P.; Wood, Chris M.

In: Journal of Experimental Biology, Vol. 204, No. 23, 01.12.2001, p. 4115-4124.

Research output: Contribution to journalArticle

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