The construction of cDNA expression library from the venom of Inimicus japonicus

Xiaoyu Jiang, Hong Bin Tu, Hui Ping Chen, Jian Wen Wei, Wen Li Yang, Wen Yan Wu, An Long Xu

Research output: Contribution to journalArticle

Abstract

A cDNA library of the venom of Inimicus japonicus was constructed. The cDNA was cloned into eukaryotical expression plasmid pcDNA3.0. SMART™ protocol was used for cDNA library construction and bioinformatics analysis was carried out. 94 novel EST clones were obtained from 150 sequences in the library, of which there were 35 full-length clones, including cytolysin genes, short neurotoxin gene, C-type lectin gene, macrophage migration inhibitory factor gene and so on. Most of those genes were reported for the first time in Inimicus japonicus. Further studies on those genes and large scales of sequencing in the library are going on, which may be helpful to make clear the components of Inimicus japonicus venom and understand the function of those proteins at molecular level.

Original languageEnglish (US)
Pages (from-to)424-428
Number of pages5
JournalProgress in Biochemistry and Biophysics
Volume29
Issue number3
StatePublished - Jun 2002
Externally publishedYes

Fingerprint

Venoms
Gene Library
Complementary DNA
Genes
Libraries
Clone Cells
Macrophage Migration-Inhibitory Factors
C-Type Lectins
Perforin
Expressed Sequence Tags
Neurotoxins
Bioinformatics
Computational Biology
Plasmids
Proteins

Keywords

  • cDNA expression library
  • Inimicus japonicus
  • Venom

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics

Cite this

Jiang, X., Tu, H. B., Chen, H. P., Wei, J. W., Yang, W. L., Wu, W. Y., & Xu, A. L. (2002). The construction of cDNA expression library from the venom of Inimicus japonicus. Progress in Biochemistry and Biophysics, 29(3), 424-428.

The construction of cDNA expression library from the venom of Inimicus japonicus. / Jiang, Xiaoyu; Tu, Hong Bin; Chen, Hui Ping; Wei, Jian Wen; Yang, Wen Li; Wu, Wen Yan; Xu, An Long.

In: Progress in Biochemistry and Biophysics, Vol. 29, No. 3, 06.2002, p. 424-428.

Research output: Contribution to journalArticle

Jiang, X, Tu, HB, Chen, HP, Wei, JW, Yang, WL, Wu, WY & Xu, AL 2002, 'The construction of cDNA expression library from the venom of Inimicus japonicus', Progress in Biochemistry and Biophysics, vol. 29, no. 3, pp. 424-428.
Jiang, Xiaoyu ; Tu, Hong Bin ; Chen, Hui Ping ; Wei, Jian Wen ; Yang, Wen Li ; Wu, Wen Yan ; Xu, An Long. / The construction of cDNA expression library from the venom of Inimicus japonicus. In: Progress in Biochemistry and Biophysics. 2002 ; Vol. 29, No. 3. pp. 424-428.
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