The construction of cDNA expression library from the tentacles of Sagartia rosea

Wen Hua Liu; Yi Liang Wang; Hui Ping Chen; Xiao Yu Jiang; Hong Bin Tu; Jian Wen Wei; Wen Lie Peng; An Long Xu

The construction of cDNA expression library from the tentacles of Sagartia rosea

Wen Hua Liu, Yi Liang Wang, Hui Ping Chen, Xiao Yu Jiang, Hong Bin Tu, Jian Wen Wei, Wen Lie Peng, An Long Xu

Research output: Contribution to journal › Article › peer-review

Abstract

A cDNA expression library of the tentacles of Sagartia rosea was constructed. The cDNA was cloned into eukaryotical expression plasmid pcDNA3. SMART protocol was used for cDNA library construction and bioinformatics analysis was carried out. 71 novel EST clones were obtained from 130 sequences in the library, of which there were 21 full-length clones, including cytolysin genes, flourescent protein, ubiquinol-cytochrome C reductase gene, elongation factor, ferritin gene riboflavin kinase gene, ribosomal protein. This provides a base for further investigating their biological activity and application.

Original language	English (US)
Pages (from-to)	749-753
Number of pages	5
Journal	Sheng wu gong cheng xue bao = Chinese journal of biotechnology
Volume	18
Issue number	6
State	Published - Nov 2002
Externally published	Yes

ASJC Scopus subject areas

Biotechnology
Applied Microbiology and Biotechnology

Cite this

The construction of cDNA expression library from the tentacles of Sagartia rosea. / Liu, Wen Hua; Wang, Yi Liang; Chen, Hui Ping; Jiang, Xiao Yu; Tu, Hong Bin; Wei, Jian Wen; Peng, Wen Lie; Xu, An Long.

In: Sheng wu gong cheng xue bao = Chinese journal of biotechnology, Vol. 18, No. 6, 11.2002, p. 749-753.

Research output: Contribution to journal › Article › peer-review

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abstract = "A cDNA expression library of the tentacles of Sagartia rosea was constructed. The cDNA was cloned into eukaryotical expression plasmid pcDNA3. SMART protocol was used for cDNA library construction and bioinformatics analysis was carried out. 71 novel EST clones were obtained from 130 sequences in the library, of which there were 21 full-length clones, including cytolysin genes, flourescent protein, ubiquinol-cytochrome C reductase gene, elongation factor, ferritin gene riboflavin kinase gene, ribosomal protein. This provides a base for further investigating their biological activity and application.",

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AU - Jiang, Xiao Yu

AU - Tu, Hong Bin

AU - Wei, Jian Wen

AU - Peng, Wen Lie

AU - Xu, An Long

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N2 - A cDNA expression library of the tentacles of Sagartia rosea was constructed. The cDNA was cloned into eukaryotical expression plasmid pcDNA3. SMART protocol was used for cDNA library construction and bioinformatics analysis was carried out. 71 novel EST clones were obtained from 130 sequences in the library, of which there were 21 full-length clones, including cytolysin genes, flourescent protein, ubiquinol-cytochrome C reductase gene, elongation factor, ferritin gene riboflavin kinase gene, ribosomal protein. This provides a base for further investigating their biological activity and application.

AB - A cDNA expression library of the tentacles of Sagartia rosea was constructed. The cDNA was cloned into eukaryotical expression plasmid pcDNA3. SMART protocol was used for cDNA library construction and bioinformatics analysis was carried out. 71 novel EST clones were obtained from 130 sequences in the library, of which there were 21 full-length clones, including cytolysin genes, flourescent protein, ubiquinol-cytochrome C reductase gene, elongation factor, ferritin gene riboflavin kinase gene, ribosomal protein. This provides a base for further investigating their biological activity and application.

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The construction of cDNA expression library from the tentacles of Sagartia rosea

Abstract

ASJC Scopus subject areas

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