Abstract
A cDNA expression library of the tentacles of Sagartia rosea was constructed. The cDNA was cloned into eukaryotical expression plasmid pcDNA3. SMART protocol was used for cDNA library construction and bioinformatics analysis was carried out. 71 novel EST clones were obtained from 130 sequences in the library, of which there were 21 full-length clones, including cytolysin genes, flourescent protein, ubiquinol-cytochrome C reductase gene, elongation factor, ferritin gene riboflavin kinase gene, ribosomal protein. This provides a base for further investigating their biological activity and application.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 749-753 |
| Number of pages | 5 |
| Journal | Sheng wu gong cheng xue bao = Chinese journal of biotechnology |
| Volume | 18 |
| Issue number | 6 |
| State | Published - 2002 |
| Externally published | Yes |
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The construction of cDNA expression library from the tentacles of Sagartia rosea. / Liu, Wen Hua; Wang, Yi Liang; Chen, Hui Ping; Jiang, Xiaoyu; Tu, Hong Bin; Wei, Jian Wen; Peng, Wen Lie; Xu, An Long.
In: Sheng wu gong cheng xue bao = Chinese journal of biotechnology, Vol. 18, No. 6, 2002, p. 749-753.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - The construction of cDNA expression library from the tentacles of Sagartia rosea
AU - Liu, Wen Hua
AU - Wang, Yi Liang
AU - Chen, Hui Ping
AU - Jiang, Xiaoyu
AU - Tu, Hong Bin
AU - Wei, Jian Wen
AU - Peng, Wen Lie
AU - Xu, An Long
PY - 2002
Y1 - 2002
N2 - A cDNA expression library of the tentacles of Sagartia rosea was constructed. The cDNA was cloned into eukaryotical expression plasmid pcDNA3. SMART protocol was used for cDNA library construction and bioinformatics analysis was carried out. 71 novel EST clones were obtained from 130 sequences in the library, of which there were 21 full-length clones, including cytolysin genes, flourescent protein, ubiquinol-cytochrome C reductase gene, elongation factor, ferritin gene riboflavin kinase gene, ribosomal protein. This provides a base for further investigating their biological activity and application.
AB - A cDNA expression library of the tentacles of Sagartia rosea was constructed. The cDNA was cloned into eukaryotical expression plasmid pcDNA3. SMART protocol was used for cDNA library construction and bioinformatics analysis was carried out. 71 novel EST clones were obtained from 130 sequences in the library, of which there were 21 full-length clones, including cytolysin genes, flourescent protein, ubiquinol-cytochrome C reductase gene, elongation factor, ferritin gene riboflavin kinase gene, ribosomal protein. This provides a base for further investigating their biological activity and application.
UR - http://www.scopus.com/inward/record.url?scp=0038166668&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0038166668&partnerID=8YFLogxK
M3 - Article
C2 - 12674649
AN - SCOPUS:0038166668
VL - 18
SP - 749
EP - 753
JO - Shengwu Gongcheng Xuebao/Chinese Journal of Biotechnology
JF - Shengwu Gongcheng Xuebao/Chinese Journal of Biotechnology
SN - 1000-3061
IS - 6
ER -