The construction of cDNA expression library from the tentacles of Sagartia rosea

Wen Hua Liu, Yi Liang Wang, Hui Ping Chen, Xiaoyu Jiang, Hong Bin Tu, Jian Wen Wei, Wen Lie Peng, An Long Xu

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

A cDNA expression library of the tentacles of Sagartia rosea was constructed. The cDNA was cloned into eukaryotical expression plasmid pcDNA3. SMART protocol was used for cDNA library construction and bioinformatics analysis was carried out. 71 novel EST clones were obtained from 130 sequences in the library, of which there were 21 full-length clones, including cytolysin genes, flourescent protein, ubiquinol-cytochrome C reductase gene, elongation factor, ferritin gene riboflavin kinase gene, ribosomal protein. This provides a base for further investigating their biological activity and application.

Original languageEnglish (US)
Pages (from-to)749-753
Number of pages5
JournalSheng wu gong cheng xue bao = Chinese journal of biotechnology
Volume18
Issue number6
StatePublished - 2002
Externally publishedYes

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riboflavin kinase
Gene Library
Clone Cells
Cytochrome Reductases
Peptide Elongation Factors
Perforin
Ribosomal Proteins
Expressed Sequence Tags
Ferritins
Computational Biology
Genes
Libraries
Proteins
Plasmids
Complementary DNA
ubiquinol

Cite this

Liu, W. H., Wang, Y. L., Chen, H. P., Jiang, X., Tu, H. B., Wei, J. W., ... Xu, A. L. (2002). The construction of cDNA expression library from the tentacles of Sagartia rosea. Sheng wu gong cheng xue bao = Chinese journal of biotechnology, 18(6), 749-753.

The construction of cDNA expression library from the tentacles of Sagartia rosea. / Liu, Wen Hua; Wang, Yi Liang; Chen, Hui Ping; Jiang, Xiaoyu; Tu, Hong Bin; Wei, Jian Wen; Peng, Wen Lie; Xu, An Long.

In: Sheng wu gong cheng xue bao = Chinese journal of biotechnology, Vol. 18, No. 6, 2002, p. 749-753.

Research output: Contribution to journalArticle

Liu, WH, Wang, YL, Chen, HP, Jiang, X, Tu, HB, Wei, JW, Peng, WL & Xu, AL 2002, 'The construction of cDNA expression library from the tentacles of Sagartia rosea', Sheng wu gong cheng xue bao = Chinese journal of biotechnology, vol. 18, no. 6, pp. 749-753.
Liu, Wen Hua ; Wang, Yi Liang ; Chen, Hui Ping ; Jiang, Xiaoyu ; Tu, Hong Bin ; Wei, Jian Wen ; Peng, Wen Lie ; Xu, An Long. / The construction of cDNA expression library from the tentacles of Sagartia rosea. In: Sheng wu gong cheng xue bao = Chinese journal of biotechnology. 2002 ; Vol. 18, No. 6. pp. 749-753.
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AU - Wei, Jian Wen

AU - Peng, Wen Lie

AU - Xu, An Long

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