The connexin43 gene is responsive to oestrogen

W. Yu, Gerhard Dahl, R. Werner

Research output: Contribution to journalArticle

164 Citations (Scopus)

Abstract

Connexin43 is the major protein of gap junctions in heart and smooth muscle including the myometrium. Molecular cloning of the connexin43 gene reveals a similar organization to that exhibited by other connexin genes: the 5' untranslated region is interrupted by an 8.5 kilobase intron. The promoter region preceding the first exon contains a TATA box and AP-1 and AP-2 sites. In addition, a series of half-palindromic oestrogen response elements is present in this region. When this promoter is linked to the reporter gene luciferase, it drives the expression of luciferase constitutively in HeLa cells transfected with this luciferase-connexin43 promoter fusion construct. When the same cells are cotransfected with oestrogen receptor cDNA, an upregulation of luciferase expression by oestrogen occurs in a cell-specific manner.

Original languageEnglish
Pages (from-to)125-132
Number of pages8
JournalProceedings of the Royal Society B: Biological Sciences
Volume255
Issue number1343
StatePublished - Mar 11 1994

Fingerprint

Connexin 43
luciferase
Luciferases
estrogens
Estrogens
Genes
connexins
Connexins
gene
promoter regions
genes
myometrium
TATA box
TATA Box
Myometrium
Cloning
response elements
5' Untranslated Regions
5' untranslated regions
Transcription Factor AP-1

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Agricultural and Biological Sciences (miscellaneous)

Cite this

The connexin43 gene is responsive to oestrogen. / Yu, W.; Dahl, Gerhard; Werner, R.

In: Proceedings of the Royal Society B: Biological Sciences, Vol. 255, No. 1343, 11.03.1994, p. 125-132.

Research output: Contribution to journalArticle

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