The concentration of LH-RH receptors in the nuclei of pancreatic cancer cells. Effect of (D-Trp6)LH-RH on tumor-bearing Syrian golden hamsters.

B. Szende, A. Csikós, K. Szepeshazi, J. D. Neill, J. J. Mulchahey, G. Halmos, K. Lapis, Andrew V Schally

Research output: Contribution to journalArticle

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Abstract

LH-RH analogs cause some inhibition of growth of pancreatic cancers. Syrian golden hamsters bearing chemically induced pancreatic cancers were treated with [D-Trp6]LH-RH for 3 d before sacrifice. LH-RH receptors were localized by electron-microscopic immunohistochemistry in the tumor cells of both treated and untreated hamsters. [D-Trp6]LH-RH treatment resulted in a marked increase in the concentration of LH-RH receptors in the nuclei. The dissociation constants (Kd) and the maximal binding capacity of the LH-RH receptors (Bmax), measured by radioreceptor assay, were higher in the nuclei of the pancreatic tumor cells of hamsters treated with [D-Trp6]LH-RH than in the untreated controls. Pancreatic cells of tumor-free hamsters did not show immunostaining for LH-RH receptors. A possible correlation between the increase in the concentration of the LH-RH receptors in the nuclei and the tumor growth-inhibiting activity of [D-Trp6]LH-RH is suggested.

Original languageEnglish
Pages (from-to)201-207
Number of pages7
JournalReceptor
Volume4
Issue number4
StatePublished - Dec 1 1994
Externally publishedYes

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Bearings (structural)
LH Receptors
Mesocricetus
Pancreatic Neoplasms
Gonadotropin-Releasing Hormone
Tumors
Cells
Neoplasms
Cricetinae
Radioligand Assay
Growth
Assays

ASJC Scopus subject areas

  • Biochemistry
  • Pharmacology

Cite this

The concentration of LH-RH receptors in the nuclei of pancreatic cancer cells. Effect of (D-Trp6)LH-RH on tumor-bearing Syrian golden hamsters. / Szende, B.; Csikós, A.; Szepeshazi, K.; Neill, J. D.; Mulchahey, J. J.; Halmos, G.; Lapis, K.; Schally, Andrew V.

In: Receptor, Vol. 4, No. 4, 01.12.1994, p. 201-207.

Research output: Contribution to journalArticle

Szende, B, Csikós, A, Szepeshazi, K, Neill, JD, Mulchahey, JJ, Halmos, G, Lapis, K & Schally, AV 1994, 'The concentration of LH-RH receptors in the nuclei of pancreatic cancer cells. Effect of (D-Trp6)LH-RH on tumor-bearing Syrian golden hamsters.', Receptor, vol. 4, no. 4, pp. 201-207.
Szende B, Csikós A, Szepeshazi K, Neill JD, Mulchahey JJ, Halmos G et al. The concentration of LH-RH receptors in the nuclei of pancreatic cancer cells. Effect of (D-Trp6)LH-RH on tumor-bearing Syrian golden hamsters. Receptor. 1994 Dec 1;4(4):201-207.
Szende, B. ; Csikós, A. ; Szepeshazi, K. ; Neill, J. D. ; Mulchahey, J. J. ; Halmos, G. ; Lapis, K. ; Schally, Andrew V. / The concentration of LH-RH receptors in the nuclei of pancreatic cancer cells. Effect of (D-Trp6)LH-RH on tumor-bearing Syrian golden hamsters. In: Receptor. 1994 ; Vol. 4, No. 4. pp. 201-207.
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AU - Halmos, G.

AU - Lapis, K.

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AB - LH-RH analogs cause some inhibition of growth of pancreatic cancers. Syrian golden hamsters bearing chemically induced pancreatic cancers were treated with [D-Trp6]LH-RH for 3 d before sacrifice. LH-RH receptors were localized by electron-microscopic immunohistochemistry in the tumor cells of both treated and untreated hamsters. [D-Trp6]LH-RH treatment resulted in a marked increase in the concentration of LH-RH receptors in the nuclei. The dissociation constants (Kd) and the maximal binding capacity of the LH-RH receptors (Bmax), measured by radioreceptor assay, were higher in the nuclei of the pancreatic tumor cells of hamsters treated with [D-Trp6]LH-RH than in the untreated controls. Pancreatic cells of tumor-free hamsters did not show immunostaining for LH-RH receptors. A possible correlation between the increase in the concentration of the LH-RH receptors in the nuclei and the tumor growth-inhibiting activity of [D-Trp6]LH-RH is suggested.

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