The complete tetramerization of rabbit muscle glycogen phosphorylase b by AMP and salt. An analytical ultracentrifuge study

Sáid A. Assaf; Adel A. Yunis

doi:10.1016/0006-291X(71)90510-9

The complete tetramerization of rabbit muscle glycogen phosphorylase b by AMP and salt. An analytical ultracentrifuge study

Sáid A. Assaf, Adel A. Yunis

Medicine

Research output: Contribution to journal › Article › peer-review

12 Scopus citations

Abstract

The association of dimetric rabbit phosphorylase b to tetrameric form was studied. The results indicate that transformation from dimer to tetramer in the presence of 0.2 M sodium fluoride and 0.04 M 2-mercapto-ethanol (at 20° at pH 6.8 or 7.5) is influenced by 5′-AMP as follows: 1. (1){A figure is presented} 2. (2){A figure is presented}. In the absence of sodium fluoride, complete tetramerization of the enzyme with 5 × 10^-3 M AMP was not possible.

Original language	English (US)
Pages (from-to)	865-870
Number of pages	6
Journal	Biochemical and biophysical research communications
Volume	42
Issue number	5
DOIs	https://doi.org/10.1016/0006-291X(71)90510-9
State	Published - Mar 5 1971

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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title = "The complete tetramerization of rabbit muscle glycogen phosphorylase b by AMP and salt. An analytical ultracentrifuge study",

abstract = "The association of dimetric rabbit phosphorylase b to tetrameric form was studied. The results indicate that transformation from dimer to tetramer in the presence of 0.2 M sodium fluoride and 0.04 M 2-mercapto-ethanol (at 20° at pH 6.8 or 7.5) is influenced by 5′-AMP as follows: 1. (1){A figure is presented} 2. (2){A figure is presented}. In the absence of sodium fluoride, complete tetramerization of the enzyme with 5 × 10-3 M AMP was not possible.",

author = "Assaf, {S{\'a}id A.} and Yunis, {Adel A.}",

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AU - Assaf, Sáid A.

AU - Yunis, Adel A.

PY - 1971/3/5

Y1 - 1971/3/5

N2 - The association of dimetric rabbit phosphorylase b to tetrameric form was studied. The results indicate that transformation from dimer to tetramer in the presence of 0.2 M sodium fluoride and 0.04 M 2-mercapto-ethanol (at 20° at pH 6.8 or 7.5) is influenced by 5′-AMP as follows: 1. (1){A figure is presented} 2. (2){A figure is presented}. In the absence of sodium fluoride, complete tetramerization of the enzyme with 5 × 10-3 M AMP was not possible.

AB - The association of dimetric rabbit phosphorylase b to tetrameric form was studied. The results indicate that transformation from dimer to tetramer in the presence of 0.2 M sodium fluoride and 0.04 M 2-mercapto-ethanol (at 20° at pH 6.8 or 7.5) is influenced by 5′-AMP as follows: 1. (1){A figure is presented} 2. (2){A figure is presented}. In the absence of sodium fluoride, complete tetramerization of the enzyme with 5 × 10-3 M AMP was not possible.

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