The complete tetramerization of rabbit muscle glycogen phosphorylase b by AMP and salt. An analytical ultracentrifuge study

Sáid A. Assaf, Adel A. Yunis

Research output: Contribution to journalArticle

12 Scopus citations

Abstract

The association of dimetric rabbit phosphorylase b to tetrameric form was studied. The results indicate that transformation from dimer to tetramer in the presence of 0.2 M sodium fluoride and 0.04 M 2-mercapto-ethanol (at 20° at pH 6.8 or 7.5) is influenced by 5′-AMP as follows: 1. (1){A figure is presented} 2. (2){A figure is presented}. In the absence of sodium fluoride, complete tetramerization of the enzyme with 5 × 10-3 M AMP was not possible.

Original languageEnglish (US)
Pages (from-to)865-870
Number of pages6
JournalBiochemical and biophysical research communications
Volume42
Issue number5
DOIs
StatePublished - Mar 5 1971

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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