The comparative molecular biology of HTLV-I and HTLV-II.

Joseph D Rosenblatt, W. Wachsman, K. Shimotohno, D. Slamon, I. S. Chen

Research output: Contribution to journalArticle

Abstract

The human T-cell leukemia viruses (HTLV) I and II, have been implicated in naturally occurring T-cell malignancies in man. We are engaged in ongoing comparative studies of HTLV-I and HTLV-II in our laboratory. We have isolated a replication-competent clone of HTLV-II, as well as several defective HTLV-II proviruses from the Mo-T hairy-cell leukemia line. HTLV-II is able to transform normal T-lymphocytes, and can replicate in both T- and B-cell lines. We have devised a convenient system allowing direct transfection of the HTLV-II genome into a B-cell line, followed by selection of HTLV-infected cells. Transfection studies with HTLV-II indicate that the virus can replicate in lymphoid cells, but not in fibroblasts. Transfection of recombinant constructs demonstrates that the viral long terminal repeat (LTR) can function as a promoter in lymphoid cells, but not in fibroblasts, suggesting a role for the viral LTR in conferring target cell specificity. Structural similarities between the HTLV-I and HTLV-II LTRs, including the presence of similar repeated base sequences in the U3 region, may account for this LTR specificity. The X region of HTLV-II has been sequenced and compared to that of HTLV-I. We have identified an mRNA species encoded by the major open reading frames in the X region of HTLV-I and HTLV-II and have located the splice acceptor site. Using antisera generated to short peptide sequences encoded by X, we have identified specific X-encoded viral proteins in HTLV infected cells.(ABSTRACT TRUNCATED AT 250 WORDS)

Original languageEnglish
Pages (from-to)177-185
Number of pages9
JournalPrincess Takamatsu symposia
Volume15
StatePublished - Dec 1 1984
Externally publishedYes

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Human T-lymphotropic virus 2
Human T-lymphotropic virus 1
Molecular Biology
Terminal Repeat Sequences
Deltaretrovirus
Transfection
Cell Line
B-Lymphocytes
Fibroblasts
Lymphocytes
T-Lymphocytes
T-Cell Leukemia
Hairy Cell Leukemia
Proviruses
RNA Splice Sites
Viral Proteins
Open Reading Frames
Immune Sera
Clone Cells

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Rosenblatt, J. D., Wachsman, W., Shimotohno, K., Slamon, D., & Chen, I. S. (1984). The comparative molecular biology of HTLV-I and HTLV-II. Princess Takamatsu symposia, 15, 177-185.

The comparative molecular biology of HTLV-I and HTLV-II. / Rosenblatt, Joseph D; Wachsman, W.; Shimotohno, K.; Slamon, D.; Chen, I. S.

In: Princess Takamatsu symposia, Vol. 15, 01.12.1984, p. 177-185.

Research output: Contribution to journalArticle

Rosenblatt, JD, Wachsman, W, Shimotohno, K, Slamon, D & Chen, IS 1984, 'The comparative molecular biology of HTLV-I and HTLV-II.', Princess Takamatsu symposia, vol. 15, pp. 177-185.
Rosenblatt JD, Wachsman W, Shimotohno K, Slamon D, Chen IS. The comparative molecular biology of HTLV-I and HTLV-II. Princess Takamatsu symposia. 1984 Dec 1;15:177-185.
Rosenblatt, Joseph D ; Wachsman, W. ; Shimotohno, K. ; Slamon, D. ; Chen, I. S. / The comparative molecular biology of HTLV-I and HTLV-II. In: Princess Takamatsu symposia. 1984 ; Vol. 15. pp. 177-185.
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