Abstract
The effects of intracellular Mg2+ on Ca2+-activated isometric tension generation in rat cardiac muscle fibers and frog skeletal muscle were compared. The membranous sarcolemmal barrier was removed from rat cardiac muscle fibers by mechanical disruption and from frog skeletal muscle by skinning. Tension was recorded in the fibers in bathing solutions of different Ca2+ concentrations and either 5×10-5 M or 1×10-3 M Mg2+ concentration (the same concentrations used in a previous study on single skinned frog skeletal muscle fibers [3]). The amount of Ca2+ required to activate the muscle increased with Mg2+ concentration for both rat ventricular muscle and frog skeletal muscle. These data indicate that intracellular Mg2+ concentration could strongly modulate Ca2+-activated tension in cardiac muscle and that very similar molecular mechanisms are responsible for Ca2+-activated tension in rat ventricular muscle and frog skeletal muscle. The possible sites of action of Mg2+ on Ca2+-activated tension are discussed.
| Original language | English |
|---|---|
| Pages (from-to) | 195-201 |
| Number of pages | 7 |
| Journal | Pflügers Archiv European Journal of Physiology |
| Volume | 358 |
| Issue number | 3 |
| DOIs | |
| State | Published - Sep 1 1975 |
| Externally published | Yes |
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Keywords
- Ca Sensitivity
- Ca-Activated Isometric Tension
- Intracellular Ca
- Intracellular Mg
- Removal of Sarcolemma
ASJC Scopus subject areas
- Physiology
Cite this
The comparative effects of [Ca2+] and [Mg2+] on tension generation in the fibers of skinned frog skeletal muscle and mechanically disrupted rat ventricular cardiac muscle. / Kerrick, W. Glenn; Donaldson, Sue K Bolitho.
In: Pflügers Archiv European Journal of Physiology, Vol. 358, No. 3, 01.09.1975, p. 195-201.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - The comparative effects of [Ca2+] and [Mg2+] on tension generation in the fibers of skinned frog skeletal muscle and mechanically disrupted rat ventricular cardiac muscle
AU - Kerrick, W. Glenn
AU - Donaldson, Sue K Bolitho
PY - 1975/9/1
Y1 - 1975/9/1
N2 - The effects of intracellular Mg2+ on Ca2+-activated isometric tension generation in rat cardiac muscle fibers and frog skeletal muscle were compared. The membranous sarcolemmal barrier was removed from rat cardiac muscle fibers by mechanical disruption and from frog skeletal muscle by skinning. Tension was recorded in the fibers in bathing solutions of different Ca2+ concentrations and either 5×10-5 M or 1×10-3 M Mg2+ concentration (the same concentrations used in a previous study on single skinned frog skeletal muscle fibers [3]). The amount of Ca2+ required to activate the muscle increased with Mg2+ concentration for both rat ventricular muscle and frog skeletal muscle. These data indicate that intracellular Mg2+ concentration could strongly modulate Ca2+-activated tension in cardiac muscle and that very similar molecular mechanisms are responsible for Ca2+-activated tension in rat ventricular muscle and frog skeletal muscle. The possible sites of action of Mg2+ on Ca2+-activated tension are discussed.
AB - The effects of intracellular Mg2+ on Ca2+-activated isometric tension generation in rat cardiac muscle fibers and frog skeletal muscle were compared. The membranous sarcolemmal barrier was removed from rat cardiac muscle fibers by mechanical disruption and from frog skeletal muscle by skinning. Tension was recorded in the fibers in bathing solutions of different Ca2+ concentrations and either 5×10-5 M or 1×10-3 M Mg2+ concentration (the same concentrations used in a previous study on single skinned frog skeletal muscle fibers [3]). The amount of Ca2+ required to activate the muscle increased with Mg2+ concentration for both rat ventricular muscle and frog skeletal muscle. These data indicate that intracellular Mg2+ concentration could strongly modulate Ca2+-activated tension in cardiac muscle and that very similar molecular mechanisms are responsible for Ca2+-activated tension in rat ventricular muscle and frog skeletal muscle. The possible sites of action of Mg2+ on Ca2+-activated tension are discussed.
KW - Ca Sensitivity
KW - Ca-Activated Isometric Tension
KW - Intracellular Ca
KW - Intracellular Mg
KW - Removal of Sarcolemma
UR - http://www.scopus.com/inward/record.url?scp=0016863797&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0016863797&partnerID=8YFLogxK
U2 - 10.1007/BF00587216
DO - 10.1007/BF00587216
M3 - Article
C2 - 1081680
AN - SCOPUS:0016863797
VL - 358
SP - 195
EP - 201
JO - Pflugers Archiv European Journal of Physiology
JF - Pflugers Archiv European Journal of Physiology
SN - 0031-6768
IS - 3
ER -