Abstract
Signaling through the common γ chain (γc), a subunit of the receptors for IL-2, -4, -7, -9, and -15, is critical for lymphocyte development, with the IL-7/IL-7R representing one important interaction. To investigate the stages of intrathymic T cell development that are dependent on γc and to determine whether γc controls T cell development solely as a component of the IL-7R, intrathymic T cell development was compared in IL-7R α-deficient mice and anti-γc-treated chimeric mice reconstituted with bone marrow and purified pro-T cells. In the presence of anti-γc, each of four phenotypically distinguishable stages of CD4-CD8- thymocytes failed to reconstitute T cell development, suggesting that each of these subsets of pro-T cells required γc for their differentiation and/or growth. Reconstitution of anti-γc-treated chimeric mice with bone marrow from IL-7R α-deficient mice indicated that IL-7R only partially contributed to intrathymic T cell development. Furthermore, when compared with IL-7R-deficient mice, anti-γc chimeric and γc-deficient mice exhibited a distinct phenotypic pattern of pro-T cell development. Collectively, these results indicate that several γc-sharing cytokines may contribute to T cell development in the thymus and suggest that one of these cytokines may be novel.
| Original language | English |
|---|---|
| Pages (from-to) | 2592-2599 |
| Number of pages | 8 |
| Journal | Journal of Immunology |
| Volume | 158 |
| Issue number | 6 |
| State | Published - Mar 15 1997 |
Fingerprint
ASJC Scopus subject areas
- Immunology
Cite this
The Common γ-Chain of Cytokine Receptors Regulates Intrathymic T Cell Development at Multiple Stages. / He, You Wen; Nakajima, Hiroshi; Leonard, Warren J.; Adkins, Rebecca D; Malek, Thomas.
In: Journal of Immunology, Vol. 158, No. 6, 15.03.1997, p. 2592-2599.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - The Common γ-Chain of Cytokine Receptors Regulates Intrathymic T Cell Development at Multiple Stages
AU - He, You Wen
AU - Nakajima, Hiroshi
AU - Leonard, Warren J.
AU - Adkins, Rebecca D
AU - Malek, Thomas
PY - 1997/3/15
Y1 - 1997/3/15
N2 - Signaling through the common γ chain (γc), a subunit of the receptors for IL-2, -4, -7, -9, and -15, is critical for lymphocyte development, with the IL-7/IL-7R representing one important interaction. To investigate the stages of intrathymic T cell development that are dependent on γc and to determine whether γc controls T cell development solely as a component of the IL-7R, intrathymic T cell development was compared in IL-7R α-deficient mice and anti-γc-treated chimeric mice reconstituted with bone marrow and purified pro-T cells. In the presence of anti-γc, each of four phenotypically distinguishable stages of CD4-CD8- thymocytes failed to reconstitute T cell development, suggesting that each of these subsets of pro-T cells required γc for their differentiation and/or growth. Reconstitution of anti-γc-treated chimeric mice with bone marrow from IL-7R α-deficient mice indicated that IL-7R only partially contributed to intrathymic T cell development. Furthermore, when compared with IL-7R-deficient mice, anti-γc chimeric and γc-deficient mice exhibited a distinct phenotypic pattern of pro-T cell development. Collectively, these results indicate that several γc-sharing cytokines may contribute to T cell development in the thymus and suggest that one of these cytokines may be novel.
AB - Signaling through the common γ chain (γc), a subunit of the receptors for IL-2, -4, -7, -9, and -15, is critical for lymphocyte development, with the IL-7/IL-7R representing one important interaction. To investigate the stages of intrathymic T cell development that are dependent on γc and to determine whether γc controls T cell development solely as a component of the IL-7R, intrathymic T cell development was compared in IL-7R α-deficient mice and anti-γc-treated chimeric mice reconstituted with bone marrow and purified pro-T cells. In the presence of anti-γc, each of four phenotypically distinguishable stages of CD4-CD8- thymocytes failed to reconstitute T cell development, suggesting that each of these subsets of pro-T cells required γc for their differentiation and/or growth. Reconstitution of anti-γc-treated chimeric mice with bone marrow from IL-7R α-deficient mice indicated that IL-7R only partially contributed to intrathymic T cell development. Furthermore, when compared with IL-7R-deficient mice, anti-γc chimeric and γc-deficient mice exhibited a distinct phenotypic pattern of pro-T cell development. Collectively, these results indicate that several γc-sharing cytokines may contribute to T cell development in the thymus and suggest that one of these cytokines may be novel.
UR - http://www.scopus.com/inward/record.url?scp=0031569097&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0031569097&partnerID=8YFLogxK
M3 - Article
C2 - 9058791
AN - SCOPUS:0031569097
VL - 158
SP - 2592
EP - 2599
JO - Journal of Immunology
JF - Journal of Immunology
SN - 0022-1767
IS - 6
ER -