TY - JOUR
T1 - The Common γ-Chain of Cytokine Receptors Regulates Intrathymic T Cell Development at Multiple Stages
AU - He, You Wen
AU - Nakajima, Hiroshi
AU - Leonard, Warren J.
AU - Adkins, Becky
AU - Malek, Thomas R.
PY - 1997/3/15
Y1 - 1997/3/15
N2 - Signaling through the common γ chain (γc), a subunit of the receptors for IL-2, -4, -7, -9, and -15, is critical for lymphocyte development, with the IL-7/IL-7R representing one important interaction. To investigate the stages of intrathymic T cell development that are dependent on γc and to determine whether γc controls T cell development solely as a component of the IL-7R, intrathymic T cell development was compared in IL-7R α-deficient mice and anti-γc-treated chimeric mice reconstituted with bone marrow and purified pro-T cells. In the presence of anti-γc, each of four phenotypically distinguishable stages of CD4-CD8- thymocytes failed to reconstitute T cell development, suggesting that each of these subsets of pro-T cells required γc for their differentiation and/or growth. Reconstitution of anti-γc-treated chimeric mice with bone marrow from IL-7R α-deficient mice indicated that IL-7R only partially contributed to intrathymic T cell development. Furthermore, when compared with IL-7R-deficient mice, anti-γc chimeric and γc-deficient mice exhibited a distinct phenotypic pattern of pro-T cell development. Collectively, these results indicate that several γc-sharing cytokines may contribute to T cell development in the thymus and suggest that one of these cytokines may be novel.
AB - Signaling through the common γ chain (γc), a subunit of the receptors for IL-2, -4, -7, -9, and -15, is critical for lymphocyte development, with the IL-7/IL-7R representing one important interaction. To investigate the stages of intrathymic T cell development that are dependent on γc and to determine whether γc controls T cell development solely as a component of the IL-7R, intrathymic T cell development was compared in IL-7R α-deficient mice and anti-γc-treated chimeric mice reconstituted with bone marrow and purified pro-T cells. In the presence of anti-γc, each of four phenotypically distinguishable stages of CD4-CD8- thymocytes failed to reconstitute T cell development, suggesting that each of these subsets of pro-T cells required γc for their differentiation and/or growth. Reconstitution of anti-γc-treated chimeric mice with bone marrow from IL-7R α-deficient mice indicated that IL-7R only partially contributed to intrathymic T cell development. Furthermore, when compared with IL-7R-deficient mice, anti-γc chimeric and γc-deficient mice exhibited a distinct phenotypic pattern of pro-T cell development. Collectively, these results indicate that several γc-sharing cytokines may contribute to T cell development in the thymus and suggest that one of these cytokines may be novel.
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M3 - Article
C2 - 9058791
AN - SCOPUS:0031569097
VL - 158
SP - 2592
EP - 2599
JO - Journal of Immunology
JF - Journal of Immunology
SN - 0022-1767
IS - 6
ER -