The cloned C-terminal region of a Trypanosoma cruzi P ribosomal protein harbors two antigenic determinants

M. J. Levin; R. Rossi; G. Levitus; E. Mesri; S. Bonnefoy; N. Kerner; M. Hontebeyrie-Joskowicz

doi:10.1016/0165-2478(90)90038-R

The cloned C-terminal region of a Trypanosoma cruzi P ribosomal protein harbors two antigenic determinants

M. J. Levin, R. Rossi, G. Levitus, E. Mesri, S. Bonnefoy, N. Kerner, M. Hontebeyrie-Joskowicz

Research output: Contribution to journal › Article

15 Scopus citations

Abstract

A DNA strategy was designed to characterize the antigenic site(s) within a γgt11 cloned 35-amino-acid antigenic peptide, identified with antibodies from patients with chronic Chagas' heart disease (cChHD) and systematic lupus erythematosus (SLE) as the C-terminal portion of a Trypanosoma cruzi P ribosomal protein. The 198-bp cDNA insert was digested with AluI, resulting in two DNA segments that were recloned in γgt11. To identify specific antigenic determinants, the recombinant phage and the purified recombinant antigens were probed with sera from clinically characterized subjects. Chronic ChHD and SLE sera defined a linear epitope common to both diseases within the 15 C-terminal residues of the parasite P ribosomal protein. It is also shown that the cloned 35-amino-acid peptide contained an antigenic site unique to cChHD.

Original language	English (US)
Pages (from-to)	69-73
Number of pages	5
Journal	Immunology Letters
Volume	24
Issue number	1
DOIs	https://doi.org/10.1016/0165-2478(90)90038-R
State	Published - Jan 1 1990
Externally published	Yes

Keywords

Chagas' heart disease
P ribosomal protein
Recombinant antigen
Systematic lupus erythematosus

ASJC Scopus subject areas

Immunology
Immunology and Allergy

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Levin, M. J., Rossi, R., Levitus, G., Mesri, E., Bonnefoy, S., Kerner, N., & Hontebeyrie-Joskowicz, M. (1990). The cloned C-terminal region of a Trypanosoma cruzi P ribosomal protein harbors two antigenic determinants. Immunology Letters, 24(1), 69-73. https://doi.org/10.1016/0165-2478(90)90038-R

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abstract = "A DNA strategy was designed to characterize the antigenic site(s) within a γgt11 cloned 35-amino-acid antigenic peptide, identified with antibodies from patients with chronic Chagas' heart disease (cChHD) and systematic lupus erythematosus (SLE) as the C-terminal portion of a Trypanosoma cruzi P ribosomal protein. The 198-bp cDNA insert was digested with AluI, resulting in two DNA segments that were recloned in γgt11. To identify specific antigenic determinants, the recombinant phage and the purified recombinant antigens were probed with sera from clinically characterized subjects. Chronic ChHD and SLE sera defined a linear epitope common to both diseases within the 15 C-terminal residues of the parasite P ribosomal protein. It is also shown that the cloned 35-amino-acid peptide contained an antigenic site unique to cChHD.",

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AU - Levin, M. J.

AU - Rossi, R.

AU - Levitus, G.

AU - Mesri, E.

AU - Bonnefoy, S.

AU - Kerner, N.

AU - Hontebeyrie-Joskowicz, M.

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N2 - A DNA strategy was designed to characterize the antigenic site(s) within a γgt11 cloned 35-amino-acid antigenic peptide, identified with antibodies from patients with chronic Chagas' heart disease (cChHD) and systematic lupus erythematosus (SLE) as the C-terminal portion of a Trypanosoma cruzi P ribosomal protein. The 198-bp cDNA insert was digested with AluI, resulting in two DNA segments that were recloned in γgt11. To identify specific antigenic determinants, the recombinant phage and the purified recombinant antigens were probed with sera from clinically characterized subjects. Chronic ChHD and SLE sera defined a linear epitope common to both diseases within the 15 C-terminal residues of the parasite P ribosomal protein. It is also shown that the cloned 35-amino-acid peptide contained an antigenic site unique to cChHD.

AB - A DNA strategy was designed to characterize the antigenic site(s) within a γgt11 cloned 35-amino-acid antigenic peptide, identified with antibodies from patients with chronic Chagas' heart disease (cChHD) and systematic lupus erythematosus (SLE) as the C-terminal portion of a Trypanosoma cruzi P ribosomal protein. The 198-bp cDNA insert was digested with AluI, resulting in two DNA segments that were recloned in γgt11. To identify specific antigenic determinants, the recombinant phage and the purified recombinant antigens were probed with sera from clinically characterized subjects. Chronic ChHD and SLE sera defined a linear epitope common to both diseases within the 15 C-terminal residues of the parasite P ribosomal protein. It is also shown that the cloned 35-amino-acid peptide contained an antigenic site unique to cChHD.

KW - Chagas' heart disease

KW - P ribosomal protein

KW - Recombinant antigen

KW - Systematic lupus erythematosus

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