The ciliopathy gene rpgrip1l is essential for hair follicle development

Jiang Chen, Christine Laclef, Alejandra Moncayo, Elizabeth R. Snedecor, Ning Yang, Li Li, Ken Ichi Takemaru, Ralf Paus, Sylvie Schneider-Maunoury, Richard A. Clark

Research output: Contribution to journalArticle

11 Scopus citations

Abstract

The primary cilium is essential for skin morphogenesis through regulating the Notch, Wnt, and hedgehog signaling pathways. Prior studies on the functions of primary cilia in the skin were based on the investigations of genes that are essential for cilium formation. However, none of these ciliogenic genes has been linked to ciliopathy, a group of disorders caused by abnormal formation or function of cilia. To determine whether there is a genetic and molecular link between ciliopathies and skin morphogenesis, we investigated the role of RPGRIP1L, a gene mutated in Joubert (JBTS) and Meckel (MKS) syndromes, two severe forms of ciliopathy, in the context of skin development. We found that RPGRIP1L is essential for hair follicle morphogenesis. Specifically, disrupting the Rpgrip1l gene in mice resulted in reduced proliferation and differentiation of follicular keratinocytes, leading to hair follicle developmental defects. These defects were associated with significantly decreased primary cilium formation and attenuated hedgehog signaling. In contrast, we found that hair follicle induction and polarization and the development of interfollicular epidermis were unaffected. This study indicates that RPGRIP1L, a ciliopathy gene, is essential for hair follicle morphogenesis likely through regulating primary cilia formation and the hedgehog signaling pathway.

Original languageEnglish (US)
Pages (from-to)701-709
Number of pages9
JournalJournal of Investigative Dermatology
Volume135
Issue number3
DOIs
StatePublished - Mar 12 2015

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ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Dermatology
  • Cell Biology

Cite this

Chen, J., Laclef, C., Moncayo, A., Snedecor, E. R., Yang, N., Li, L., Takemaru, K. I., Paus, R., Schneider-Maunoury, S., & Clark, R. A. (2015). The ciliopathy gene rpgrip1l is essential for hair follicle development. Journal of Investigative Dermatology, 135(3), 701-709. https://doi.org/10.1038/jid.2014.483