The chromatin remodeling factor BRG1 stimulates nucleotide excision repair by facilitating recruitment of XPC to sites of DNA damage

Ling Zhang, Qian Zhang, Kristi Jones, Mausam Patel, Feng Gong

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

BRG1 is a catalytic subunit of the human SWI/SNF-like BAF chromatin remodeling complexes. Recent findings have shown that inactivation of BRG1 sensitizes mammalian cells to various DNA damaging agents, including ultraviolet (UV) and ionizing radiation. However, it is unclear whether BRG1 facilitates nucleotide excision repair (NER). Here we show that re-expression of BRG1 in cells lacking endogenous BRG1 expression stimulates nucleotide excision repair of UV induced DNA damage. Using a micropore UV radiation technique, we demonstrate that recruitment of the DNA damage recognition protein XPC to sites of UV lesions is disrupted when BRG1 is stably depleted. Chromatin immunoprecipitation of the endogenous DDB2 protein, which is involved in recruiting XPC to UV-induced CPDs (cyclobutane pyrimidine dimers), shows that elevated levels of BRG1 are associated with DDB2 in chromatin in response to UV radiation. Additionally, we detected slow BRG1 accumulation at sites of UV lesions. Our findings suggest that the chromatin remodeling factor BRG1 is recruited to sites of UV lesions to facilitate NER in human chromatin.

Original languageEnglish
Pages (from-to)3953-3959
Number of pages7
JournalCell Cycle
Volume8
Issue number23
StatePublished - Dec 1 2009

Fingerprint

Chromatin Assembly and Disassembly
DNA Repair
DNA Damage
Chromatin
Radiation
Pyrimidine Dimers
Chromatin Immunoprecipitation
Ionizing Radiation
Catalytic Domain
Proteins
DNA

Keywords

  • BRG1
  • DNA damage
  • Nucleotide excision repair
  • SWI/SNF
  • UV radiation

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology
  • Developmental Biology

Cite this

The chromatin remodeling factor BRG1 stimulates nucleotide excision repair by facilitating recruitment of XPC to sites of DNA damage. / Zhang, Ling; Zhang, Qian; Jones, Kristi; Patel, Mausam; Gong, Feng.

In: Cell Cycle, Vol. 8, No. 23, 01.12.2009, p. 3953-3959.

Research output: Contribution to journalArticle

Zhang, Ling ; Zhang, Qian ; Jones, Kristi ; Patel, Mausam ; Gong, Feng. / The chromatin remodeling factor BRG1 stimulates nucleotide excision repair by facilitating recruitment of XPC to sites of DNA damage. In: Cell Cycle. 2009 ; Vol. 8, No. 23. pp. 3953-3959.
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