The cholinergic response is increased in isolated ileum from gastroschisis rat model

Aline Cristina Régis, Julio Alejandro Rojas-Moscoso, Frances Lilian Lanhellas Gonçalves, Augusto Frederico Schmidt, Fabíola Zakia Mónica, Edson Antunes, Lourenço Sbragia

Research output: Contribution to journalArticlepeer-review

3 Scopus citations


Introduction Babies with gastroschisis (G) have high morbidity rate and long hospital stay due to bowel hypomotility caused by chronic exposure of the bowel to the amniotic fluid. Our aim was to evaluate the reactivity of isolated ileum in fetal rats selected for experimental gastroschisis. Method G was surgically created at 18.5 days of gestation (term = 22 days). Concentration-dependent curve to the muscarinic agonist methacholine (1-30 lM) and contractions induced by electrical field stimulation (EFS, 1-16 Hz, 50 V, 1 ms) were carried out in isolated ileum of groups control (C), sham (S) and gastroschisis (G) (n = 30). Protein expression for M3 was assessed by western blot analysis. Results The frequency and amplitude of spontaneous contractions were decreased in G (p<0.001). Methacholine produced concentration-dependent contractions being the maximal response values higher in G (p<0.01). EFSinduced frequency-dependent contractions showed 1.8 times higher in G as well as an increase of M3 expression. Conclusion The frequency and the amplitude of rhythmic contractions were reduced along with an increase in the contraction induced by mucarinic agonist and by EFS in G. These results suggest the occurrence of an adaptative supersensitivity to cholinergic response via increases in the protein expression for M3 receptor.

Original languageEnglish (US)
Pages (from-to)1015-1019
Number of pages5
JournalPediatric Surgery International
Issue number9
StatePublished - Sep 2011
Externally publishedYes


  • Fetal surgery
  • Gastroschisis
  • Gut development
  • Intestinal motility
  • Rat model

ASJC Scopus subject areas

  • Pediatrics, Perinatology, and Child Health
  • Surgery


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