The cellular uptake and covalent binding of nitroso-chloramphenicol

Thomas Murray, Adel A Yunis

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

A comparative study of the cellular transport of CAP and its nitroso derivative (NO-CAP) was carried out in Raji cells, a transformed human lymphoblastoid cell line. Both agents were concentrated by the cells by a factor of 3 (cellular/extracellular concentration ratio). The cellular uptake of NO-CAP, like that of CAP, was found to be rapid and temperature-independent. Thus the greater cytotoxicity of NO-CAP is apparently not due to an enhanced uptake of the nitroso derivative relative to CAP. In contrast to the similarity of uptake, NO-CAP becomes covalently bound to both Raji cells and freshly isolated human bone marrow cells to a much higher extent (15-fold). Also, cells previously loaded with CAP or NO-CAP retain three times as much of the nitroso compound during a 24 hr dialysis against a drug-free isotonic solution. The increased binding of NO-CAP to human hematopoietic cells attests to the greater reactivity of the p-substituted aromatic nitroso group and is consistent with the postulate that reduction products of the nitro group of CAP may be responsible for CAP-induced aplastic anemia.

Original languageEnglish (US)
Pages (from-to)396-401
Number of pages6
JournalThe Journal of laboratory and clinical medicine
Volume98
Issue number3
StatePublished - 1981

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Chloramphenicol
Cells
Nitroso Compounds
Derivatives
Isotonic Solutions
Dialysis
Cytotoxicity
Bone
Pharmaceutical Preparations
Aplastic Anemia
Bone Marrow Cells
Temperature
Cell Line

ASJC Scopus subject areas

  • Medicine(all)
  • Pathology and Forensic Medicine

Cite this

The cellular uptake and covalent binding of nitroso-chloramphenicol. / Murray, Thomas; Yunis, Adel A.

In: The Journal of laboratory and clinical medicine, Vol. 98, No. 3, 1981, p. 396-401.

Research output: Contribution to journalArticle

Murray, Thomas ; Yunis, Adel A. / The cellular uptake and covalent binding of nitroso-chloramphenicol. In: The Journal of laboratory and clinical medicine. 1981 ; Vol. 98, No. 3. pp. 396-401.
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