The C5b-6 complex

formation, isolation, and inhibition of its activity by lipoprotein and the S-protein of human serum

E. R. Podack, W. P. Kolb, H. J. Muller-Eberhard

Research output: Contribution to journalArticle

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Abstract

C5b-6 complex was generated by activation of C7-depleted serum and purified 1200-fold based on its hemolytic activity. A homogeneous bimolecular complex with an s-rate of 11.5S and a m.w. of 328,000 daltons was obtained. The electrophoretic mobility was -3.4 x 10-5 cm2/volt sec. The subunit composition was revealed by SDS-polyacrylamide electrophoresis without reduction showed two protein bands: C5b (180,000) and C6 (120,000); and with reduction three subunits: C6 (128,000), C5bα (122,000), and C5β (75,000). Immunochemical analysis confirmed complex formation between C5b and C6 and showed the presence of one of the two neoantigens expressed by the SC5b-9 complex. Sixty nanograms of C5b-6 represented 108 hemolytically effective molecules. A hemolytic efficiency of 12 to 20 C5b-6 cell-bound complexes per lytic event was calculated from the uptake of radiolabeled C5b-6. The hemolytic efficiency of C5b-6 is dependent on the cell concentration. C5b-6-initiated lysis is strongly inhibited by serum low density lipoproteins with an inhibition constant of k(i) = 34 μg/ml and by the S-protein with k(i) = 39μg/ml. Lipoproteins and S-protein from a stable, hemolytically inactive complex with nascent C5b-7. The mechanism of C5b-7 inhibition is interpreted as a competition reaction between the target cell membrane and the inhibitors for the transitory binding site in nascent C5b-7.

Original languageEnglish
Pages (from-to)1841-1848
Number of pages8
JournalJournal of Immunology
Volume120
Issue number6
StatePublished - Dec 1 1978
Externally publishedYes

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Protein S
Lipoproteins
Blood Proteins
Serum
LDL Lipoproteins
Electrophoresis
complement C5b-6 complex
Binding Sites
Cell Membrane
complement C5b-7 complex
Proteins

ASJC Scopus subject areas

  • Immunology

Cite this

The C5b-6 complex : formation, isolation, and inhibition of its activity by lipoprotein and the S-protein of human serum. / Podack, E. R.; Kolb, W. P.; Muller-Eberhard, H. J.

In: Journal of Immunology, Vol. 120, No. 6, 01.12.1978, p. 1841-1848.

Research output: Contribution to journalArticle

Podack, E. R. ; Kolb, W. P. ; Muller-Eberhard, H. J. / The C5b-6 complex : formation, isolation, and inhibition of its activity by lipoprotein and the S-protein of human serum. In: Journal of Immunology. 1978 ; Vol. 120, No. 6. pp. 1841-1848.
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