TY - JOUR
T1 - The c-fos cyclic AMP-responsive element conveys constitutive expression to a tissue-specific promoter
AU - Webster, K. A.
AU - Kedes, L.
N1 - Copyright:
Copyright 2017 Elsevier B.V., All rights reserved.
PY - 1990
Y1 - 1990
N2 - The c-fos and cardiac α-actin promoters share homologous 5′ protein binding elements that are essential for serum-inducible and tissue-specific expression, respectively. Additional elements, auxiliary proteins or factor modifications, must distinguish the individual transcriptional responses of these two promoters. An element in the c-fos basal promoter that is normally responsible for transient stimulation of the fos gene in response to Ca2+ or cyclic AMP (CRE) may be able to modulate the expression of the upstream elements. We report here that this element, when inserted into the cardiac α-actin promoter, conveys constitutive expression to this otherwise highly restricted promoter. Additional data support the proposal that the CRE binding protein creates an alternative pathway whereby upstream regulatory elements in the cardiac α-actin promoter can activate transcription in a manner which circumvents the requirement for a tissue-specific environment.
AB - The c-fos and cardiac α-actin promoters share homologous 5′ protein binding elements that are essential for serum-inducible and tissue-specific expression, respectively. Additional elements, auxiliary proteins or factor modifications, must distinguish the individual transcriptional responses of these two promoters. An element in the c-fos basal promoter that is normally responsible for transient stimulation of the fos gene in response to Ca2+ or cyclic AMP (CRE) may be able to modulate the expression of the upstream elements. We report here that this element, when inserted into the cardiac α-actin promoter, conveys constitutive expression to this otherwise highly restricted promoter. Additional data support the proposal that the CRE binding protein creates an alternative pathway whereby upstream regulatory elements in the cardiac α-actin promoter can activate transcription in a manner which circumvents the requirement for a tissue-specific environment.
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U2 - 10.1128/MCB.10.5.2402
DO - 10.1128/MCB.10.5.2402
M3 - Article
C2 - 2157971
AN - SCOPUS:0025272210
VL - 10
SP - 2402
EP - 2406
JO - Molecular and Cellular Biology
JF - Molecular and Cellular Biology
SN - 0270-7306
IS - 5
ER -