The brefeldin A-inhibited GDP/GTP exchange factor 2, a protein involved in vesicular trafficking, interacts with the β subunits of the GABA A receptors

Erik I. Charych, Wendou Yu, Celia P. Miralles, David R. Serwanski, Xuejing Li, Maria Rubio, Angel L. De Blas

Research output: Contribution to journalArticle

77 Citations (Scopus)

Abstract

We have found that the brefeldin A-inhibited GDP/GTP exchange factor 2 (BIG2) interacts with the β subunits of the γ-aminobutyric acid type-A receptor (GABAAR). BIG2 is a Sec7 domain-containing guanine nucleotide exchange factor known to be involved in vesicular and protein trafficking. The interaction between the 110 amino acid C-terminal fragment of BIG2 and the large intracellular loop of the GABAAR β subunits was revealed with a yeast two-hybrid assay. The native BIG2 and GABA ARs interact in the brain since both coprecipitated from detergent extracts with either anti-GABAAR or anti-BIG2 antibodies. In transfected human embryonic kidney cell line 293 cells, BIG2 promotes the exit of GABAARs from endoplasmic reticulum. Double label immunofluorescence of cultured hippocampal neurons and electron microscopy immunocytochemistry of rat brain tissue show that BIG2 concentrates in the trans-Golgi network. BIG2 is also present in vesicle-like structures in the dendritic cytoplasm, sometimes colocalizing with GABAARS. BIG2 is present in both inhibitory GABAergic synapses that contain GABAARs and in asymmetric excitatory synapses. The results are consistent with the hypotheses that the interaction of BIG2 with the GABAAR β subunits plays a role in the exocytosis and trafficking of assembled GABA AR to the cell surface.

Original languageEnglish (US)
Pages (from-to)173-189
Number of pages17
JournalJournal of Neurochemistry
Volume90
Issue number1
DOIs
StatePublished - Jul 2004
Externally publishedYes

Fingerprint

Brefeldin A
Guanine Nucleotide Exchange Factors
GABA-A Receptors
Guanosine Triphosphate
Synapses
gamma-Aminobutyric Acid
Brain
Aminobutyrates
trans-Golgi Network
Two-Hybrid System Techniques
HEK293 Cells
Exocytosis
Protein Transport
Endoplasmic Reticulum
Detergents
Yeast
Electron microscopy
Neurons
Fluorescent Antibody Technique
Rats

Keywords

  • γ-aminobutyric acid type-A receptor
  • ADP-ribosylation factor
  • BIG2
  • Brain
  • Sec7
  • Synapses

ASJC Scopus subject areas

  • Biochemistry
  • Cellular and Molecular Neuroscience

Cite this

The brefeldin A-inhibited GDP/GTP exchange factor 2, a protein involved in vesicular trafficking, interacts with the β subunits of the GABA A receptors. / Charych, Erik I.; Yu, Wendou; Miralles, Celia P.; Serwanski, David R.; Li, Xuejing; Rubio, Maria; De Blas, Angel L.

In: Journal of Neurochemistry, Vol. 90, No. 1, 07.2004, p. 173-189.

Research output: Contribution to journalArticle

Charych, Erik I. ; Yu, Wendou ; Miralles, Celia P. ; Serwanski, David R. ; Li, Xuejing ; Rubio, Maria ; De Blas, Angel L. / The brefeldin A-inhibited GDP/GTP exchange factor 2, a protein involved in vesicular trafficking, interacts with the β subunits of the GABA A receptors. In: Journal of Neurochemistry. 2004 ; Vol. 90, No. 1. pp. 173-189.
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