The biological importance of measuring individual variation

Research output: Contribution to journalArticle

62 Citations (Scopus)

Abstract

Functional genomics research using Fundulus heteroclitus has focused on variation among individuals because of the evolutionary importance and value of Fundulus in explaining the human condition (why individual humans are different and are affected differently by stress, disease and drugs). Among different populations and species of Fundulus, there are evolutionarily adaptive differences in gene expression. This natural variation in gene expression seems to affect cardiac metabolism because up to 81% of the variation in glucose utilization observed in isolated heart ventricles is related to specific patterns of gene expression. The surprising result from this research is that among different groups of individuals, the expression of mRNA from different metabolic pathways explains substrate-specific metabolism. For example, variation in oxidative phosphorylation mRNAs explains glucose metabolism for one group of individuals but expression of glucose metabolism genes explains this metabolism in a different group of individuals. This variation among individuals has important implications for studies using inbred strains: conclusions based on one individual or one strain will not necessarily reflect a generalized conclusion for a population or species. Finally, there are surprisingly strong positive and negative correlations among metabolic genes, both within and between pathways. These data suggest that measures of mRNA expression are meaningful, yet there is a complexity in how gene expression is related to physiological processes.

Original languageEnglish
Pages (from-to)1613-1621
Number of pages9
JournalJournal of Experimental Biology
Volume210
Issue number9
DOIs
StatePublished - May 1 2007

Fingerprint

individual variation
Fundulidae
metabolism
gene expression
Fundulus
Gene Expression
glucose
Glucose
Messenger RNA
heart ventricle
Physiological Phenomena
Fundulus heteroclitus
oxidative phosphorylation
gene
Oxidative Phosphorylation
Genomics
Metabolic Networks and Pathways
Research
Population
Genes

Keywords

  • Cardiac metabolism
  • Evolutionary selection
  • Functional genomics
  • Fundulus heteroclitus
  • Gene expression
  • Microarray
  • mRNA expression
  • Phenotypic variation

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Agricultural and Biological Sciences (miscellaneous)

Cite this

The biological importance of measuring individual variation. / Crawford, Douglas L; Oleksiak, Marjorie F.

In: Journal of Experimental Biology, Vol. 210, No. 9, 01.05.2007, p. 1613-1621.

Research output: Contribution to journalArticle

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