The biogenesis of muscle glycogen: Regulation of the activity of the autocatalytic primer protein

J. Lomako, W. M. Lomako, W. J. Whelan

Research output: Contribution to journalArticlepeer-review

12 Scopus citations

Abstract

Glycogen synthesis in rabbit muscle is initiated by an autocatalytic, self-glucosylating protein (SGP). This creates a maltosaccharide primer on itself that in turn primes glycogen synthesis. Here we describe the powerful allosteric inhibition of autocatalysis by ATP and ADP, sufficient, at the physiological concentration of ATP in muscle, to inhibit autocatalysis. We also examined inhibition of self-glucosylation by analogues of the substrate UDPglucose. One of them, UDPxylose, acts as an alternative substrate and serves to block glucosylation. An improved purification procedure for the SGP is also described.

Original languageEnglish (US)
Pages (from-to)251-254
Number of pages4
JournalBioFactors
Volume2
Issue number4
StatePublished - 1990

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Medicine
  • Clinical Biochemistry

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