The binary protein-protein interaction landscape of escherichia coli

Seesandra V. Rajagopala, Patricia Sikorski, Ashwani Kumar, Roberto Mosca, James Vlasblom, Roland Arnold, Jonathan Franca-Koh, Suman B. Pakala, Sadhna Phanse, Arnaud Ceol, Roman Häuser, Gabriella Siszler, Stefan Wuchty, Andrew Emili, Mohan Babu, Patrick Aloy, Rembert Pieper, Peter Uetz

Research output: Contribution to journalArticle

98 Citations (Scopus)

Abstract

Efforts to map the Escherichia coli interactome have identified several hundred macromolecular complexes, but direct binary protein-protein interactions (PPIs) have not been surveyed on a large scale. Here we performed yeast two-hybrid screens of 3,305 baits against 3,606 preys (â ̂1/470% of the E. coli proteome) in duplicate to generate a map of 2,234 interactions, which approximately doubles the number of known binary PPIs in E. coli. Integration of binary PPI and genetic-interaction data revealed functional dependencies among components involved in cellular processes, including envelope integrity, flagellum assembly and protein quality control. Many of the binary interactions that we could map in multiprotein complexes were informative regarding internal topology of complexes and indicated that interactions in complexes are substantially more conserved than those interactions connecting different complexes. This resource will be useful for inferring bacterial gene function and provides a draft reference of the basic physical wiring network of this evolutionarily important model microbe.

Original languageEnglish (US)
Pages (from-to)285-290
Number of pages6
JournalNature Biotechnology
Volume32
Issue number3
DOIs
StatePublished - 2014

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Escherichia coli
Proteins
Macromolecular Substances
Bacterial Genes
Multiprotein Complexes
Flagella
Electric wiring
Proteome
Quality Control
Yeast
Quality control
Genes
Yeasts
Topology

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Biotechnology
  • Molecular Medicine
  • Bioengineering
  • Biomedical Engineering
  • Medicine(all)

Cite this

Rajagopala, S. V., Sikorski, P., Kumar, A., Mosca, R., Vlasblom, J., Arnold, R., ... Uetz, P. (2014). The binary protein-protein interaction landscape of escherichia coli. Nature Biotechnology, 32(3), 285-290. https://doi.org/10.1038/nbt.2831

The binary protein-protein interaction landscape of escherichia coli. / Rajagopala, Seesandra V.; Sikorski, Patricia; Kumar, Ashwani; Mosca, Roberto; Vlasblom, James; Arnold, Roland; Franca-Koh, Jonathan; Pakala, Suman B.; Phanse, Sadhna; Ceol, Arnaud; Häuser, Roman; Siszler, Gabriella; Wuchty, Stefan; Emili, Andrew; Babu, Mohan; Aloy, Patrick; Pieper, Rembert; Uetz, Peter.

In: Nature Biotechnology, Vol. 32, No. 3, 2014, p. 285-290.

Research output: Contribution to journalArticle

Rajagopala, SV, Sikorski, P, Kumar, A, Mosca, R, Vlasblom, J, Arnold, R, Franca-Koh, J, Pakala, SB, Phanse, S, Ceol, A, Häuser, R, Siszler, G, Wuchty, S, Emili, A, Babu, M, Aloy, P, Pieper, R & Uetz, P 2014, 'The binary protein-protein interaction landscape of escherichia coli', Nature Biotechnology, vol. 32, no. 3, pp. 285-290. https://doi.org/10.1038/nbt.2831
Rajagopala SV, Sikorski P, Kumar A, Mosca R, Vlasblom J, Arnold R et al. The binary protein-protein interaction landscape of escherichia coli. Nature Biotechnology. 2014;32(3):285-290. https://doi.org/10.1038/nbt.2831
Rajagopala, Seesandra V. ; Sikorski, Patricia ; Kumar, Ashwani ; Mosca, Roberto ; Vlasblom, James ; Arnold, Roland ; Franca-Koh, Jonathan ; Pakala, Suman B. ; Phanse, Sadhna ; Ceol, Arnaud ; Häuser, Roman ; Siszler, Gabriella ; Wuchty, Stefan ; Emili, Andrew ; Babu, Mohan ; Aloy, Patrick ; Pieper, Rembert ; Uetz, Peter. / The binary protein-protein interaction landscape of escherichia coli. In: Nature Biotechnology. 2014 ; Vol. 32, No. 3. pp. 285-290.
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