The BE (2)-M17 neuroblastoma cell line synthesizes and secretes corticotropin-releasing factor

John W. Kasckow, David G. Parkes, Michael J. Owens, Mark D. Stipetic, Jin Hee Han, Charles Nemeroff, Wylie W. Vale

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

The BE (2)-M17 human neuroblastoma has previously been show to express corticotropin-releasing factor (CRF) mRNA following retinoic acid treatment. It is demonstrated in this report that both cell extracts and cell incubation medium of retinoic acid-treated BE (2)-M17 cells were shown to contain CRF-like immunoreactivity (CRF-LI) by RIA. CRF-LI secretion and content were also dose-dependently increased by forskolin. In addition, cell extracts were applied to a C18 Vydac column and peak CRF-LI from the collected fractions was shown to coincide in time of elution with peak immunoreactivity seen with oxidized synthetic CRF standard. Thus, in containing the CRF peptide, the BE (2)-M17 cells are useful models for further study of CRF cellular and genetic regulation.

Original languageEnglish
Pages (from-to)159-162
Number of pages4
JournalBrain Research
Volume654
Issue number1
DOIs
StatePublished - Aug 15 1994
Externally publishedYes

Fingerprint

Corticotropin-Releasing Hormone
Neuroblastoma
Cell Line
Tretinoin
Cell Extracts
Colforsin
Messenger RNA
Peptides

Keywords

  • BE (2)-M17
  • Forskolin
  • HPLC
  • Neuroblastoma
  • orticotropin-releasing factor
  • RIA

ASJC Scopus subject areas

  • Developmental Biology
  • Molecular Biology
  • Clinical Neurology
  • Neuroscience(all)

Cite this

Kasckow, J. W., Parkes, D. G., Owens, M. J., Stipetic, M. D., Han, J. H., Nemeroff, C., & Vale, W. W. (1994). The BE (2)-M17 neuroblastoma cell line synthesizes and secretes corticotropin-releasing factor. Brain Research, 654(1), 159-162. https://doi.org/10.1016/0006-8993(94)91583-0

The BE (2)-M17 neuroblastoma cell line synthesizes and secretes corticotropin-releasing factor. / Kasckow, John W.; Parkes, David G.; Owens, Michael J.; Stipetic, Mark D.; Han, Jin Hee; Nemeroff, Charles; Vale, Wylie W.

In: Brain Research, Vol. 654, No. 1, 15.08.1994, p. 159-162.

Research output: Contribution to journalArticle

Kasckow, JW, Parkes, DG, Owens, MJ, Stipetic, MD, Han, JH, Nemeroff, C & Vale, WW 1994, 'The BE (2)-M17 neuroblastoma cell line synthesizes and secretes corticotropin-releasing factor', Brain Research, vol. 654, no. 1, pp. 159-162. https://doi.org/10.1016/0006-8993(94)91583-0
Kasckow, John W. ; Parkes, David G. ; Owens, Michael J. ; Stipetic, Mark D. ; Han, Jin Hee ; Nemeroff, Charles ; Vale, Wylie W. / The BE (2)-M17 neuroblastoma cell line synthesizes and secretes corticotropin-releasing factor. In: Brain Research. 1994 ; Vol. 654, No. 1. pp. 159-162.
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