The Bag Method for Islet Cell Infusion

David Baidal, Tatiana Froud, Jacqueline V. Ferreira, Aisha Khan, Rodolfo Alejandro, Camillo Ricordi

Research output: Contribution to journalArticle

71 Citations (Scopus)

Abstract

As islet cell transplantation gains increasing interest following results published by the Edmonton group, results that have been successfully reproduced by several centers nationwide and abroad, the need of guidelines to standardize the procedure becomes highly important. We detail the key steps of the infusion procedure utilizing a closed gravity fed bag system utilized at our institution since 1990, which consists of a 600-ml transfer bag and a 150-ml rinse bag connected via sterile tubing. The use of gravity allows for a control rate of infusion as well as providing a safety mechanism through natural reduction of flow that parallels any increase in portal pressure, therefore allowing the operator to prevent precipitous pressure rises. Reports on significant rise in portal pressures during islet cell infusion as well as portal vein thrombosis have been published. Infusion at these centers was carried out using a syringe method. Using our technique, portal vein thrombosis (partial or complete) was not detected in any of the infusions performed at our institution. This method may be of assistance to minimize some of the observed complications associated with islet transplant procedures and has now been adapted by most centers performing clinical islet transplantation.

Original languageEnglish
Pages (from-to)809-813
Number of pages5
JournalCell Transplantation
Volume12
Issue number7
StatePublished - Nov 24 2003

Fingerprint

Islets of Langerhans
Portal Pressure
Islets of Langerhans Transplantation
Gravitation
Portal Vein
Thrombosis
Syringes
Transplants
Parallel flow
Tubing
Cell Transplantation
Guidelines
Safety
Pressure

Keywords

  • Bag method
  • Islet cell infusion
  • Standardization of guidelines

ASJC Scopus subject areas

  • Cell Biology
  • Transplantation

Cite this

Baidal, D., Froud, T., Ferreira, J. V., Khan, A., Alejandro, R., & Ricordi, C. (2003). The Bag Method for Islet Cell Infusion. Cell Transplantation, 12(7), 809-813.

The Bag Method for Islet Cell Infusion. / Baidal, David; Froud, Tatiana; Ferreira, Jacqueline V.; Khan, Aisha; Alejandro, Rodolfo; Ricordi, Camillo.

In: Cell Transplantation, Vol. 12, No. 7, 24.11.2003, p. 809-813.

Research output: Contribution to journalArticle

Baidal, D, Froud, T, Ferreira, JV, Khan, A, Alejandro, R & Ricordi, C 2003, 'The Bag Method for Islet Cell Infusion', Cell Transplantation, vol. 12, no. 7, pp. 809-813.
Baidal D, Froud T, Ferreira JV, Khan A, Alejandro R, Ricordi C. The Bag Method for Islet Cell Infusion. Cell Transplantation. 2003 Nov 24;12(7):809-813.
Baidal, David ; Froud, Tatiana ; Ferreira, Jacqueline V. ; Khan, Aisha ; Alejandro, Rodolfo ; Ricordi, Camillo. / The Bag Method for Islet Cell Infusion. In: Cell Transplantation. 2003 ; Vol. 12, No. 7. pp. 809-813.
@article{6db09d99fc72427c900b20a98073614b,
title = "The Bag Method for Islet Cell Infusion",
abstract = "As islet cell transplantation gains increasing interest following results published by the Edmonton group, results that have been successfully reproduced by several centers nationwide and abroad, the need of guidelines to standardize the procedure becomes highly important. We detail the key steps of the infusion procedure utilizing a closed gravity fed bag system utilized at our institution since 1990, which consists of a 600-ml transfer bag and a 150-ml rinse bag connected via sterile tubing. The use of gravity allows for a control rate of infusion as well as providing a safety mechanism through natural reduction of flow that parallels any increase in portal pressure, therefore allowing the operator to prevent precipitous pressure rises. Reports on significant rise in portal pressures during islet cell infusion as well as portal vein thrombosis have been published. Infusion at these centers was carried out using a syringe method. Using our technique, portal vein thrombosis (partial or complete) was not detected in any of the infusions performed at our institution. This method may be of assistance to minimize some of the observed complications associated with islet transplant procedures and has now been adapted by most centers performing clinical islet transplantation.",
keywords = "Bag method, Islet cell infusion, Standardization of guidelines",
author = "David Baidal and Tatiana Froud and Ferreira, {Jacqueline V.} and Aisha Khan and Rodolfo Alejandro and Camillo Ricordi",
year = "2003",
month = "11",
day = "24",
language = "English",
volume = "12",
pages = "809--813",
journal = "Cell Transplantation",
issn = "0963-6897",
publisher = "Cognizant Communication Corporation",
number = "7",

}

TY - JOUR

T1 - The Bag Method for Islet Cell Infusion

AU - Baidal, David

AU - Froud, Tatiana

AU - Ferreira, Jacqueline V.

AU - Khan, Aisha

AU - Alejandro, Rodolfo

AU - Ricordi, Camillo

PY - 2003/11/24

Y1 - 2003/11/24

N2 - As islet cell transplantation gains increasing interest following results published by the Edmonton group, results that have been successfully reproduced by several centers nationwide and abroad, the need of guidelines to standardize the procedure becomes highly important. We detail the key steps of the infusion procedure utilizing a closed gravity fed bag system utilized at our institution since 1990, which consists of a 600-ml transfer bag and a 150-ml rinse bag connected via sterile tubing. The use of gravity allows for a control rate of infusion as well as providing a safety mechanism through natural reduction of flow that parallels any increase in portal pressure, therefore allowing the operator to prevent precipitous pressure rises. Reports on significant rise in portal pressures during islet cell infusion as well as portal vein thrombosis have been published. Infusion at these centers was carried out using a syringe method. Using our technique, portal vein thrombosis (partial or complete) was not detected in any of the infusions performed at our institution. This method may be of assistance to minimize some of the observed complications associated with islet transplant procedures and has now been adapted by most centers performing clinical islet transplantation.

AB - As islet cell transplantation gains increasing interest following results published by the Edmonton group, results that have been successfully reproduced by several centers nationwide and abroad, the need of guidelines to standardize the procedure becomes highly important. We detail the key steps of the infusion procedure utilizing a closed gravity fed bag system utilized at our institution since 1990, which consists of a 600-ml transfer bag and a 150-ml rinse bag connected via sterile tubing. The use of gravity allows for a control rate of infusion as well as providing a safety mechanism through natural reduction of flow that parallels any increase in portal pressure, therefore allowing the operator to prevent precipitous pressure rises. Reports on significant rise in portal pressures during islet cell infusion as well as portal vein thrombosis have been published. Infusion at these centers was carried out using a syringe method. Using our technique, portal vein thrombosis (partial or complete) was not detected in any of the infusions performed at our institution. This method may be of assistance to minimize some of the observed complications associated with islet transplant procedures and has now been adapted by most centers performing clinical islet transplantation.

KW - Bag method

KW - Islet cell infusion

KW - Standardization of guidelines

UR - http://www.scopus.com/inward/record.url?scp=0242574517&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0242574517&partnerID=8YFLogxK

M3 - Article

C2 - 14653627

AN - SCOPUS:0242574517

VL - 12

SP - 809

EP - 813

JO - Cell Transplantation

JF - Cell Transplantation

SN - 0963-6897

IS - 7

ER -