The bacterial protein YopJ abrogates multiple signal transduction pathways that converge on the transcription factor creb

Lisa K. Meijer, Kurt Schesser, Hans Wolf-Watz, Paolo Sassone-Corsi, Sven Pettersson

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

Bacterially encoded proteins are known to affect eukaryotic signalling pathways and thus cell growth and differentiation. The enteric pathogen Yersinia pseudotuberculosis (YP) can translocate Yersinia outer proteins (Yops) into eukaryotic cells. Recently, MKK proteins have been identified as tentative targets of YopJ-mediated inhibition of ligand receptor-dependent signal transduction in mammalian cells. These results prompted us to assess whether multiple signal transduction pathways and their down-stream target genes would also be subject to regulation by YopJ. Here, we show that YopJ effectively blocks the lipopolysaccharide (LPS) receptor, the interleukin (IL)-1β receptor and the UVC-induced activation of the transcription receptor cAMP response element-binding protein (CREB). In addition, by abrogating the phosphorylation of CREB and thus activating protein (AP)-1- dependent transcription, YopJ can block LPS-induced clonal expansion that is associated with an adaptive immune response. Thus, YopJ interferes with multiple pathways converging on the transcription factor CREB. Our data are discussed in the context of YopJ acting as an antagonist to circumvent innate and adaptive immune responses at multiple levels.

Original languageEnglish
Pages (from-to)231-238
Number of pages8
JournalCellular Microbiology
Volume2
Issue number3
DOIs
StatePublished - Aug 23 2000
Externally publishedYes

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Signal transduction
Bacterial Proteins
Cyclic AMP Response Element-Binding Protein
Signal Transduction
Transcription Factors
Adaptive Immunity
Transcription
Proteins
CD14 Antigens
Yersinia pseudotuberculosis
Yersinia
Phosphorylation
Interleukin-1 Receptors
Cell growth
Eukaryotic Cells
Pathogens
Innate Immunity
Transcriptional Activation
Lipopolysaccharides
Cell Differentiation

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Microbiology

Cite this

The bacterial protein YopJ abrogates multiple signal transduction pathways that converge on the transcription factor creb. / Meijer, Lisa K.; Schesser, Kurt; Wolf-Watz, Hans; Sassone-Corsi, Paolo; Pettersson, Sven.

In: Cellular Microbiology, Vol. 2, No. 3, 23.08.2000, p. 231-238.

Research output: Contribution to journalArticle

Meijer, Lisa K. ; Schesser, Kurt ; Wolf-Watz, Hans ; Sassone-Corsi, Paolo ; Pettersson, Sven. / The bacterial protein YopJ abrogates multiple signal transduction pathways that converge on the transcription factor creb. In: Cellular Microbiology. 2000 ; Vol. 2, No. 3. pp. 231-238.
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