The A3 adenosine receptor mediates cell spreading, reorganization of actin cytoskeleton, and distribution of Bcl-x(L)

Studies in human astroglioma cells

Maria P. Abbracchio, Gabriella Rainaidi, Anna Maria Giammarioli, Stefania Ceruti, Roberta Brambilla, Flaminio Cattabeni, Daniela Barbieri, Claudio Franceschi, Kenneth A. Jacobson, Walter Malorni

Research output: Contribution to journalArticle

79 Citations (Scopus)

Abstract

The pathophysiological role of the adenosine A3 receptor in the central nervous system is largely unknown. We have investigated the effects of the selective A3 receptor agonist 2-chloro-N6-(3-iodobenzyl)-adenosine, CI-IB-MECA, in cells of the astroglial lineage (human astrocytoma ADF cells). A marked reorganization of the cytoskeleton, with appearance of stress fibers and numerous cell protrusions, was found following exposure of cells to low (nM) concentrations of CI-IB-MECA. These 'trophic' effects were accompanied by induction of the expression of Rho, a small GTP-binding protein, which was virtually absent in control cells, and by changes of the intracellular distribution of the antiapoptotic protein Bcl-x(L), that, in agonist-exposed cells, became specifically associated to cell protrusions. This is the first demonstration that the intracellular organization of Bcl-x(L) can be modulated by the activation of a G-protein-coupled membrane receptor, such as the A3 adenosine receptor. Moreover, modulation of the astrocytic cytoskeleton by adenosine may have intriguing implications in both nervous system development and in the response of the brain to trauma and ischemia.

Original languageEnglish
Pages (from-to)297-304
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume241
Issue number2
DOIs
StatePublished - Dec 18 1997
Externally publishedYes

Fingerprint

Adenosine A3 Receptors
Astrocytoma
Neurology
Actin Cytoskeleton
GTP-Binding Proteins
Adenosine
Actins
Brain
Demonstrations
Chemical activation
Modulation
Cytoskeleton
Membranes
Fibers
Stress Fibers
rho GTP-Binding Proteins
Proteins
Cell Lineage
G-Protein-Coupled Receptors
Brain Ischemia

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

The A3 adenosine receptor mediates cell spreading, reorganization of actin cytoskeleton, and distribution of Bcl-x(L) : Studies in human astroglioma cells. / Abbracchio, Maria P.; Rainaidi, Gabriella; Giammarioli, Anna Maria; Ceruti, Stefania; Brambilla, Roberta; Cattabeni, Flaminio; Barbieri, Daniela; Franceschi, Claudio; Jacobson, Kenneth A.; Malorni, Walter.

In: Biochemical and Biophysical Research Communications, Vol. 241, No. 2, 18.12.1997, p. 297-304.

Research output: Contribution to journalArticle

Abbracchio, Maria P. ; Rainaidi, Gabriella ; Giammarioli, Anna Maria ; Ceruti, Stefania ; Brambilla, Roberta ; Cattabeni, Flaminio ; Barbieri, Daniela ; Franceschi, Claudio ; Jacobson, Kenneth A. ; Malorni, Walter. / The A3 adenosine receptor mediates cell spreading, reorganization of actin cytoskeleton, and distribution of Bcl-x(L) : Studies in human astroglioma cells. In: Biochemical and Biophysical Research Communications. 1997 ; Vol. 241, No. 2. pp. 297-304.
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