Abstract
The pathophysiological role of the adenosine A3 receptor in the central nervous system is largely unknown. We have investigated the effects of the selective A3 receptor agonist 2-chloro-N6-(3-iodobenzyl)-adenosine, CI-IB-MECA, in cells of the astroglial lineage (human astrocytoma ADF cells). A marked reorganization of the cytoskeleton, with appearance of stress fibers and numerous cell protrusions, was found following exposure of cells to low (nM) concentrations of CI-IB-MECA. These 'trophic' effects were accompanied by induction of the expression of Rho, a small GTP-binding protein, which was virtually absent in control cells, and by changes of the intracellular distribution of the antiapoptotic protein Bcl-x(L), that, in agonist-exposed cells, became specifically associated to cell protrusions. This is the first demonstration that the intracellular organization of Bcl-x(L) can be modulated by the activation of a G-protein-coupled membrane receptor, such as the A3 adenosine receptor. Moreover, modulation of the astrocytic cytoskeleton by adenosine may have intriguing implications in both nervous system development and in the response of the brain to trauma and ischemia.
| Original language | English |
|---|---|
| Pages (from-to) | 297-304 |
| Number of pages | 8 |
| Journal | Biochemical and Biophysical Research Communications |
| Volume | 241 |
| Issue number | 2 |
| DOIs | |
| State | Published - Dec 18 1997 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Biochemistry
- Biophysics
- Molecular Biology
Cite this
The A3 adenosine receptor mediates cell spreading, reorganization of actin cytoskeleton, and distribution of Bcl-x(L) : Studies in human astroglioma cells. / Abbracchio, Maria P.; Rainaidi, Gabriella; Giammarioli, Anna Maria; Ceruti, Stefania; Brambilla, Roberta; Cattabeni, Flaminio; Barbieri, Daniela; Franceschi, Claudio; Jacobson, Kenneth A.; Malorni, Walter.
In: Biochemical and Biophysical Research Communications, Vol. 241, No. 2, 18.12.1997, p. 297-304.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - The A3 adenosine receptor mediates cell spreading, reorganization of actin cytoskeleton, and distribution of Bcl-x(L)
T2 - Studies in human astroglioma cells
AU - Abbracchio, Maria P.
AU - Rainaidi, Gabriella
AU - Giammarioli, Anna Maria
AU - Ceruti, Stefania
AU - Brambilla, Roberta
AU - Cattabeni, Flaminio
AU - Barbieri, Daniela
AU - Franceschi, Claudio
AU - Jacobson, Kenneth A.
AU - Malorni, Walter
PY - 1997/12/18
Y1 - 1997/12/18
N2 - The pathophysiological role of the adenosine A3 receptor in the central nervous system is largely unknown. We have investigated the effects of the selective A3 receptor agonist 2-chloro-N6-(3-iodobenzyl)-adenosine, CI-IB-MECA, in cells of the astroglial lineage (human astrocytoma ADF cells). A marked reorganization of the cytoskeleton, with appearance of stress fibers and numerous cell protrusions, was found following exposure of cells to low (nM) concentrations of CI-IB-MECA. These 'trophic' effects were accompanied by induction of the expression of Rho, a small GTP-binding protein, which was virtually absent in control cells, and by changes of the intracellular distribution of the antiapoptotic protein Bcl-x(L), that, in agonist-exposed cells, became specifically associated to cell protrusions. This is the first demonstration that the intracellular organization of Bcl-x(L) can be modulated by the activation of a G-protein-coupled membrane receptor, such as the A3 adenosine receptor. Moreover, modulation of the astrocytic cytoskeleton by adenosine may have intriguing implications in both nervous system development and in the response of the brain to trauma and ischemia.
AB - The pathophysiological role of the adenosine A3 receptor in the central nervous system is largely unknown. We have investigated the effects of the selective A3 receptor agonist 2-chloro-N6-(3-iodobenzyl)-adenosine, CI-IB-MECA, in cells of the astroglial lineage (human astrocytoma ADF cells). A marked reorganization of the cytoskeleton, with appearance of stress fibers and numerous cell protrusions, was found following exposure of cells to low (nM) concentrations of CI-IB-MECA. These 'trophic' effects were accompanied by induction of the expression of Rho, a small GTP-binding protein, which was virtually absent in control cells, and by changes of the intracellular distribution of the antiapoptotic protein Bcl-x(L), that, in agonist-exposed cells, became specifically associated to cell protrusions. This is the first demonstration that the intracellular organization of Bcl-x(L) can be modulated by the activation of a G-protein-coupled membrane receptor, such as the A3 adenosine receptor. Moreover, modulation of the astrocytic cytoskeleton by adenosine may have intriguing implications in both nervous system development and in the response of the brain to trauma and ischemia.
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UR - http://www.scopus.com/inward/citedby.url?scp=0031577677&partnerID=8YFLogxK
U2 - 10.1006/bbrc.1997.7705
DO - 10.1006/bbrc.1997.7705
M3 - Article
VL - 241
SP - 297
EP - 304
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 2
ER -