The apical submembrane cytoskeleton participates in the organization of the apical pole in epithelial cells

Pedro J Salas, Marcelo L. Rodriguez, Ana L. Viciana, Dora E. Vega-Salas, Hans Peter Hauri

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Abstract

In a previous publication (Rodriguez, M.L., M. Brignoni, and P. J. I. Salas, 1994, J. Cell Sci. 107: 3145-3151), we described the existence of a terminal web-like structure in nonbrush border cells, which comprises a specifically apical cytokeratin, presumably cytokeratin 19. In the present study we confirmed the apical distribution of cytokeratin 19 and expanded that observation to other epithelial cells in tissue culture and in vivo. In tissue culture, subconfluent cell stocks under continuous treatment with two different 21-mer phosphorothioate oligodeoxy nucleotides that targeted cytokeratin 19 mRNA enabled us to obtain confluent monolayers with a partial (40-70%) and transitory reduction in this protein. The expression of other cytoskeletal proteins was undisturbed. This downregulation of cytokeratin 19 resulted in (a) decrease in the number of microvilli; (b) disorganization of the apical (but not lateral or basal) filamentous actin and abnormal apical microtubules; and (c) depletion or redistribution of apical membrane proteins as determined by differential apical-basolateral biotinylation. In fact, a subset of detergent-insoluble proteins was not expressed on the cell surface in cells with lower levels of cytokeratin 19. Apical proteins purified in the detergent phase of Triton X-114 (typically integral membrane proteins) and those differentially extracted in Triton X-100 at 37°C or in n-octyl-β-D- glycoside at 4°C (representative of GPI-anchored proteins), appeared partially redistributed to the basolateral domain. A transmembrane apical protein, sucrase isomaltase, was found mispolarized in a subpopulation of the cells treated with antisense oligonucleotides, while the basolateral polarity of Na+- K+ ATPase was not affected. Both sucrase isomaltase and alkaline phosphatase (a GPI-anchored protein) appeared partially depolarized in A19 treated CACO-2 monolayers as determined by differential biotinylation, affinity purification, and immunoblot. These results suggest that an apical submembrane cytoskeleton of intermediate filaments is expressed in a number of epithelia, including those without a brush border, although it may not be universal. In addition, these data indicate that this structure is involved in the organization of the apical region of the cytoplasm and the apical membrane.

Original languageEnglish
Pages (from-to)359-375
Number of pages17
JournalJournal of Cell Biology
Volume137
Issue number2
DOIs
StatePublished - Apr 21 1997

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Keratin-19
Cytoskeleton
Epithelial Cells
Oligo-1,6-Glucosidase
Biotinylation
Sucrase
Proteins
Microvilli
Detergents
Membrane Proteins
Cytoskeletal Proteins
Intermediate Filaments
Antisense Oligonucleotides
Octoxynol
Glycosides
Keratins
Microtubules
Alkaline Phosphatase
Actins
Cytoplasm

ASJC Scopus subject areas

  • Cell Biology

Cite this

The apical submembrane cytoskeleton participates in the organization of the apical pole in epithelial cells. / Salas, Pedro J; Rodriguez, Marcelo L.; Viciana, Ana L.; Vega-Salas, Dora E.; Hauri, Hans Peter.

In: Journal of Cell Biology, Vol. 137, No. 2, 21.04.1997, p. 359-375.

Research output: Contribution to journalArticle

Salas, Pedro J ; Rodriguez, Marcelo L. ; Viciana, Ana L. ; Vega-Salas, Dora E. ; Hauri, Hans Peter. / The apical submembrane cytoskeleton participates in the organization of the apical pole in epithelial cells. In: Journal of Cell Biology. 1997 ; Vol. 137, No. 2. pp. 359-375.
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