The Antioxidant Effect of Exercise: A Systematic Review and Meta-Analysis

Caio Victor de Sousa, Marcelo Magalhães Sales, Thiago Santos Rosa, John Eugene Lewis, Rosangela Vieira de Andrade, Herbert Gustavo Simões

Research output: Contribution to journalReview articlepeer-review

98 Scopus citations


Background: Physical activity has been associated with reduced oxidative stress (OS) in observational studies and clinical trials. Objective: The purpose of this systematic review and meta-analysis of controlled trials was to determine the effect of physical exercise on OS parameters. Methods: We conducted a systematic review of the literature up to March 2016 that included the following databases: PubMed, SCOPUS, and Web of Science. A keyword combination referring to exercise training and OS was included as part of a more thorough search process. We also manually searched the reference lists of the articles. From an initial 1573 references, we included 30 controlled trials (1346 participants) in the qualitative analysis, 19 of which were included in the meta-analysis. All trials were conducted in humans and had at least one exercise intervention and a paired control group. Using a standardized protocol, two investigators independently abstracted data on study design, sample size, participant characteristics, intervention, follow-up duration, outcomes, and quantitative data for the meta-analysis. Thus, the investigators independently assigned quality scores with a methodological quality assessment (MQA). Results: The agreement level between the reviewers was 85.3 %. Discrepancies were solved in a consensus meeting. The MQA showed a total score in the quality index between 40 and 90 % and a mean quality of 55 %. Further, in a random-effects model, data from each trial were pooled and weighted by the inverse of the total variance. Physical training was associated with a significant reduction in pro-oxidant parameters (standard mean difference [SMD] –1.08; 95 % confidence interval [CI] –1.57 to –0.58; p < 0.001) and an increase in antioxidant capacity (SMD 1.45; 95 % CI 0.83–2.06; p < 0.001). Conclusion: The pooled analysis revealed that regardless of intensity, volume, type of exercise, and studied population, the antioxidant indicators tended to increase and pro-oxidant indicators tended to decrease after training. Therefore, we conclude that exercise training seems to induce an antioxidant effect. Thus, it is suggested that people practice some kind of exercise to balance the redox state, regardless of their health status, to improve health-related outcomes.

Original languageEnglish (US)
Pages (from-to)277-293
Number of pages17
JournalSports Medicine
Issue number2
StatePublished - Feb 1 2017

ASJC Scopus subject areas

  • Orthopedics and Sports Medicine
  • Physical Therapy, Sports Therapy and Rehabilitation


Dive into the research topics of 'The Antioxidant Effect of Exercise: A Systematic Review and Meta-Analysis'. Together they form a unique fingerprint.

Cite this