The accessory function of murine intercellular adhesion molecule-1 in T lymphocyte activation

Contributions of adhesion and co-activation

Peter Kuhlman, Vincent T. Moy, Bridget A. Lollo, Adrienne A. Brian

Research output: Contribution to journalArticle

172 Citations (Scopus)

Abstract

These studies demonstrate that the murine intercellular adhesion molecule-1 (ICAM-1) performs at least two roles in enhancing T cell activation. These two roles are evident in both of our experimental systems: with ICAM-1 expressed on the surface of transfected fibroblast cells, and with purified ICAM-1 immobilized on plastic. First, as has been documented by many investigators, ICAM-1 mediates adhesion between ICAM-1- and lymphocyte function-associated Ag-1 (LFA-1)-bearing cells. This adhesive interaction occurs even in the absence of T cell stimulation, although it is increased by addition of phorbol ester and calcium ionophore. Although ICAM-1 expression does markedly increase intercellular adhesion, the increase is significantly less than the improvement ICAM-1 expression makes in the Ag-presenting ability of MHC class II-transfected fibroblast cells. We have investigated whether this difference is due to LFA-1-mediated signaling, and we present data that demonstrates that although ICAM-1 does not deliver costimulatory signals required for T cell activation, the interaction of LFA-1 with ICAM-1 does synergize with TCR-transduced signals. This synergy is observed for ICAM-1 on live and on chemically fixed accessory cells, and for purified ICAM-1 molecules, but in all cases occurs only when the ICAM-1 and the TCR ligands are on the same surface. Finally, when the ICAM-1 is present on the surface of accessory cells, it enhances T cell activation by changing the Ag dose-dependence of the T cell, but when ICAM-1 and CD3 mAb are coimmobilized, ICAM-1 increases the peak response of the T cell without affecting the dose dependence of the response.

Original languageEnglish
Pages (from-to)1773-1782
Number of pages10
JournalJournal of Immunology
Volume146
Issue number6
StatePublished - Mar 15 1991
Externally publishedYes

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Intercellular Adhesion Molecule-1
Lymphocyte Activation
T-Lymphocytes
Fibroblasts
Lymphocytes
Calcium Ionophores
Phorbol Esters
Cell Communication
Adhesives
Plastics

ASJC Scopus subject areas

  • Immunology

Cite this

The accessory function of murine intercellular adhesion molecule-1 in T lymphocyte activation : Contributions of adhesion and co-activation. / Kuhlman, Peter; Moy, Vincent T.; Lollo, Bridget A.; Brian, Adrienne A.

In: Journal of Immunology, Vol. 146, No. 6, 15.03.1991, p. 1773-1782.

Research output: Contribution to journalArticle

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