The 14.1 Surrogate Light Chain Promoter Has Lineage- and Stage-Restricted Activity

Mary E. Donohoe, Bonnie B Blomberg

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

The 14.1 surrogate light chain protein is expressed on human pre-B lymphocytes in association with Vpre-B and the μ Ig heavy chain to form the pre-B receptor. The 14.1 chain has also been called the lambda (λ)-like (LL) protein and is homologous to murine λ5. The 14.1(IGLL1) gene is expressed in a lineage- and stage-restricted manner. To understand the molecular mechanism of the 14.1 gene tissue- and stage-specific expression, we analyzed the 5′-flanking region and characterized the promoter for this gene. In this report, we identify two DNase I-hypersensitive sites located at 2.6 kilobases (HSS 1) and 0.2 kilobases (HSS 1) upstream of 14.1 exon 1. These hypersensitive sites are present in pre-B lymphocyte cell lines, but absent in mature B and T cell lines. We have used RNase protection analysis to localize the 5′ major transcriptional start site and rapid amplification of 5′ cDNA ends to identify multiple starl sites within the TATA-less, GC-rich 14.1 promoter. The region encompassing HSS 2 was analyzed for promoter activity. Transfection of cell lines with a series of truncated segments of the 5′ flanking region linked to the luciferase reporter gene revealed that the 14.1 promoter is lineage- and stage-specific, and we localized this activity to positions +150 to +227 relative to the 5′ major transcriptional start site.

Original languageEnglish
Pages (from-to)1681-1691
Number of pages11
JournalJournal of Immunology
Volume158
Issue number4
StatePublished - Feb 15 1997

Fingerprint

Surrogate Immunoglobulin Light Chains
B-Lymphoid Precursor Cells
5' Flanking Region
Cell Line
Genes
Immunoglobulin Heavy Chains
Deoxyribonuclease I
Ribonucleases
Luciferases
Reporter Genes
Transfection
Exons
Proteins
B-Lymphocytes
Complementary DNA
T-Lymphocytes

ASJC Scopus subject areas

  • Immunology

Cite this

The 14.1 Surrogate Light Chain Promoter Has Lineage- and Stage-Restricted Activity. / Donohoe, Mary E.; Blomberg, Bonnie B.

In: Journal of Immunology, Vol. 158, No. 4, 15.02.1997, p. 1681-1691.

Research output: Contribution to journalArticle

@article{5c2b1b0897774c2d918d7db02980a8e5,
title = "The 14.1 Surrogate Light Chain Promoter Has Lineage- and Stage-Restricted Activity",
abstract = "The 14.1 surrogate light chain protein is expressed on human pre-B lymphocytes in association with Vpre-B and the μ Ig heavy chain to form the pre-B receptor. The 14.1 chain has also been called the lambda (λ)-like (LL) protein and is homologous to murine λ5. The 14.1(IGLL1) gene is expressed in a lineage- and stage-restricted manner. To understand the molecular mechanism of the 14.1 gene tissue- and stage-specific expression, we analyzed the 5′-flanking region and characterized the promoter for this gene. In this report, we identify two DNase I-hypersensitive sites located at 2.6 kilobases (HSS 1) and 0.2 kilobases (HSS 1) upstream of 14.1 exon 1. These hypersensitive sites are present in pre-B lymphocyte cell lines, but absent in mature B and T cell lines. We have used RNase protection analysis to localize the 5′ major transcriptional start site and rapid amplification of 5′ cDNA ends to identify multiple starl sites within the TATA-less, GC-rich 14.1 promoter. The region encompassing HSS 2 was analyzed for promoter activity. Transfection of cell lines with a series of truncated segments of the 5′ flanking region linked to the luciferase reporter gene revealed that the 14.1 promoter is lineage- and stage-specific, and we localized this activity to positions +150 to +227 relative to the 5′ major transcriptional start site.",
author = "Donohoe, {Mary E.} and Blomberg, {Bonnie B}",
year = "1997",
month = "2",
day = "15",
language = "English",
volume = "158",
pages = "1681--1691",
journal = "Journal of Immunology",
issn = "0022-1767",
publisher = "American Association of Immunologists",
number = "4",

}

TY - JOUR

T1 - The 14.1 Surrogate Light Chain Promoter Has Lineage- and Stage-Restricted Activity

AU - Donohoe, Mary E.

AU - Blomberg, Bonnie B

PY - 1997/2/15

Y1 - 1997/2/15

N2 - The 14.1 surrogate light chain protein is expressed on human pre-B lymphocytes in association with Vpre-B and the μ Ig heavy chain to form the pre-B receptor. The 14.1 chain has also been called the lambda (λ)-like (LL) protein and is homologous to murine λ5. The 14.1(IGLL1) gene is expressed in a lineage- and stage-restricted manner. To understand the molecular mechanism of the 14.1 gene tissue- and stage-specific expression, we analyzed the 5′-flanking region and characterized the promoter for this gene. In this report, we identify two DNase I-hypersensitive sites located at 2.6 kilobases (HSS 1) and 0.2 kilobases (HSS 1) upstream of 14.1 exon 1. These hypersensitive sites are present in pre-B lymphocyte cell lines, but absent in mature B and T cell lines. We have used RNase protection analysis to localize the 5′ major transcriptional start site and rapid amplification of 5′ cDNA ends to identify multiple starl sites within the TATA-less, GC-rich 14.1 promoter. The region encompassing HSS 2 was analyzed for promoter activity. Transfection of cell lines with a series of truncated segments of the 5′ flanking region linked to the luciferase reporter gene revealed that the 14.1 promoter is lineage- and stage-specific, and we localized this activity to positions +150 to +227 relative to the 5′ major transcriptional start site.

AB - The 14.1 surrogate light chain protein is expressed on human pre-B lymphocytes in association with Vpre-B and the μ Ig heavy chain to form the pre-B receptor. The 14.1 chain has also been called the lambda (λ)-like (LL) protein and is homologous to murine λ5. The 14.1(IGLL1) gene is expressed in a lineage- and stage-restricted manner. To understand the molecular mechanism of the 14.1 gene tissue- and stage-specific expression, we analyzed the 5′-flanking region and characterized the promoter for this gene. In this report, we identify two DNase I-hypersensitive sites located at 2.6 kilobases (HSS 1) and 0.2 kilobases (HSS 1) upstream of 14.1 exon 1. These hypersensitive sites are present in pre-B lymphocyte cell lines, but absent in mature B and T cell lines. We have used RNase protection analysis to localize the 5′ major transcriptional start site and rapid amplification of 5′ cDNA ends to identify multiple starl sites within the TATA-less, GC-rich 14.1 promoter. The region encompassing HSS 2 was analyzed for promoter activity. Transfection of cell lines with a series of truncated segments of the 5′ flanking region linked to the luciferase reporter gene revealed that the 14.1 promoter is lineage- and stage-specific, and we localized this activity to positions +150 to +227 relative to the 5′ major transcriptional start site.

UR - http://www.scopus.com/inward/record.url?scp=0031568390&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0031568390&partnerID=8YFLogxK

M3 - Article

VL - 158

SP - 1681

EP - 1691

JO - Journal of Immunology

JF - Journal of Immunology

SN - 0022-1767

IS - 4

ER -