Introduction and hypothesis: Our aims were to correlate transforming growth factor (TGF)-β1 and elastin mRNA expression in the vagina of women and to measure the effects of TGF-β1 on vaginal smooth muscle cell (SMC) proliferation and tropoelastin production. Methods: Vaginal walls were sampled in women (n=20). TGF-β1 and elastin mRNA expression was assessed by RT-PCR. SMC cultures were performed from vaginal wall biopsies. SMC were incubated with TGF-β1, and cell proliferation was assessed by MTT-assay. Tropoelastin production was measured by the Fastin Elastin Assay. Results: There was a significant positive correlation between TGF-β1 and elastin mRNA (r=0.784, P< 0.01). SMC proliferation was significantly increased by 10 ng/mL TGF-β1 [relative cell number, mean ± SD, 198%±32% of control (P=0.01)]. Tropoelastin production was significantly increased by TGF-β1 [mean ± SD, 645%±180% of control (P=0.01)]. Conclusions: There is a positive correlation between TGF-β1 and elastin mRNA expression in the vaginal wall. In vitro, TGF-β1 increases vaginal tropoelastin production in vaginal SMC.
- Transforming growth factor β1
ASJC Scopus subject areas
- Obstetrics and Gynecology