TGFβ1: Modulation of Chondrogenic Differentiation by Cultured Periotic Mesenchymal Cells

D. A. FRENZ, T. R. VAN DE WATER

Research output: Contribution to journalArticle

5 Scopus citations

Abstract

The chondrogenic capacities of periotic mesenchyme from different developmentally staged mouse embryos were compared in high-density culture (Table 1). Isolated periotic mesenchyme from mouse embryos of 10.5 GD do not express a chondrogenic phenotype in culture. The mesenchyme appears fibroblastic in morphology and produces an extracellular matrix that does not bind Alcian blue stain (pH 1.0). When TGFβ1 is added (1 to 10 ng/ml, continuous) to cultures of 10.5 GD periotic mesenchyme, there is a striking increase in cell packing. Although the mesenchymal cells do not undergo chondrogenic differentiation, they begin to produce a matrix containing S-GAG. In micromass culture, isolated periotic mesenchyme from 11.5 GD embryos is limited in its ability to differentiate. However, when TGFβ1 (1 ng/ml, 24 h) is added to these cultures, a marked increase in chondrogenic potential is expressed by the periotic mesenchymal cells. A 60% increase in production of extracellular matrix rich in S-GAG results as compared to controls. By 12.5 GD, determination of mesenchyme to form cartilage is complete. The addition of TGFβ1 (1 ng/ml 24 h) to micromass cultures of periotic mesenchyme from 12.5 to 14.0 GD results in a marked suppression of chondrogenic differentiation. As compared to their respective controls, production of cartilage-specific S-GAG is reduced by 50 to 75%.

Original languageEnglish (US)
Pages (from-to)340-342
Number of pages3
JournalAnnals of the New York Academy of Sciences
Volume593
Issue number1
DOIs
StatePublished - Jun 1990

ASJC Scopus subject areas

  • Neuroscience(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • History and Philosophy of Science

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