TETRASPORE is required for male meiotic cytokinesis in Arabidopsis thaliana

Melissa Spielman, Daphne Preuss, Feng Lan Li, William Browne, Rod J. Scott, Hugh G. Dickinson

Research output: Contribution to journalArticle

133 Citations (Scopus)

Abstract

In flowering plants, male meiosis occurs in the microsporocyte to produce four microspores, each of which develops into a pollen grain. Here we describe four mutant alleles of TETRASPORE (TES), a gene essential for microsporocyte cytokinesis in Arabidopsis thaliana. Following failure of male meiotic cytokinesis in tes mutants, all four microspore nuclei remain within the same cytoplasm, with some completing their developmental programmes to form functional pollen nuclei. Both of the mitotic divisions seen in normal pollen development take place in tes mutants, including the asymmetric division required for the differentiation of gametes; some tes grains perform multiple asymmetric divisions in the same cytoplasm. tes pollen shows a variety of abnormalities subsequent to the cytokinetic defect, including fusion of nuclei, formation of ectopic internal walls, and disruptions to external wall patterning. In addition, ovules fertilized by tes pollen often abort, possibly because of excess paternal genomes in the endosperm. Thus tes mutants not only reveal a gene specific to male meiosis, but aid investigation of a wide range of processes in pollen development and function.

Original languageEnglish
Pages (from-to)2645-2657
Number of pages13
JournalDevelopment
Volume124
Issue number13
StatePublished - Jul 1 1997
Externally publishedYes

Fingerprint

Cytokinesis
Pollen
Arabidopsis
Meiosis
Cytoplasm
Ovule
Endosperm
Essential Genes
Germ Cells
Alleles
Genome
Genes

Keywords

  • Arabidopsis
  • Callose
  • Cytokinesis
  • Endosperm
  • Fertilization
  • Meiosis
  • Microspore
  • Pollen development
  • Pollen mitosis
  • Tetrad
  • TETRASPORE

ASJC Scopus subject areas

  • Anatomy
  • Cell Biology

Cite this

Spielman, M., Preuss, D., Li, F. L., Browne, W., Scott, R. J., & Dickinson, H. G. (1997). TETRASPORE is required for male meiotic cytokinesis in Arabidopsis thaliana. Development, 124(13), 2645-2657.

TETRASPORE is required for male meiotic cytokinesis in Arabidopsis thaliana. / Spielman, Melissa; Preuss, Daphne; Li, Feng Lan; Browne, William; Scott, Rod J.; Dickinson, Hugh G.

In: Development, Vol. 124, No. 13, 01.07.1997, p. 2645-2657.

Research output: Contribution to journalArticle

Spielman, M, Preuss, D, Li, FL, Browne, W, Scott, RJ & Dickinson, HG 1997, 'TETRASPORE is required for male meiotic cytokinesis in Arabidopsis thaliana', Development, vol. 124, no. 13, pp. 2645-2657.
Spielman M, Preuss D, Li FL, Browne W, Scott RJ, Dickinson HG. TETRASPORE is required for male meiotic cytokinesis in Arabidopsis thaliana. Development. 1997 Jul 1;124(13):2645-2657.
Spielman, Melissa ; Preuss, Daphne ; Li, Feng Lan ; Browne, William ; Scott, Rod J. ; Dickinson, Hugh G. / TETRASPORE is required for male meiotic cytokinesis in Arabidopsis thaliana. In: Development. 1997 ; Vol. 124, No. 13. pp. 2645-2657.
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