Testes exhibit elevated expression of calcitonin gene-related peptide receptor component protein

Wayne E Balkan, Edward L. Oates, Guy Howard, Bernard A. Roos

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Calcitonin gene-related peptide (CGRP) receptor component protein (RCP) is a novel protein that modulates CGRP responsiveness in a variety of cell types. Using probes based on the isolation of CGRP-RCP complementary DNA (cDNA) from a guinea pig organ of Corti cDNA library, we cloned human (h) and mouse (m) CGRP-RCP cDNAs, both of which encode 148-residue proteins that at the amino acid levels are approximately 88% identical to each other and to the 146-residue guinea pig CGRP-RCP. Northern blot analysis confirmed the presence of CGRP-RCP messenger RNA in all of the human and mouse tissues tested. In these human tissues, hCGRP-RCP messenger RNA (major band at ~3.1 kb, minor band at ~7.5 kb) was most prevalent in the testis. In the mouse, the highest abundance of CGRP-RCP RNA was clearly in the testis (major band at ~1.6 kb, minor band at ~1.1 kb). Based on this tissue distribution of RNA, we sought to identify the cells in the murine testis that contained CGRP-RCP protein. Numerous antisera generated against hCGRP-RCP, including one to recombinant hCGRP-RCP, exhibited strong immunoreactivity localized to the head region of spermatozoa. No CGRP-RCP immunoreactivity was observed in other cells at less mature stages of sperm maturation, in Sertoli or interstitial (Leydig) cells, or in human spermatozoa. Murine epididymal (mature) spermatozoa exhibited CGRP-RCP immunoreactivity identical to that of testicular spermatozoa. Spermatozoa that underwent an experimentally induced acrosome reaction (acrosomal discharge) lost their CGRP-RCP immunoreactivity. Therefore, it appears that CGRP-RCP is associated with the acrosome, suggesting that it may play an important role in reproduction.

Original languageEnglish
Pages (from-to)1459-1469
Number of pages11
JournalEndocrinology
Volume140
Issue number3
StatePublished - Mar 9 1999

Fingerprint

Calcitonin Gene-Related Peptide Receptors
Testis
Proteins
Spermatozoa
Complementary DNA
Guinea Pigs
RNA
Sperm Maturation
Acrosome Reaction
Organ of Corti
Acrosome
Messenger RNA
Leydig Cells
Calcitonin Gene-Related Peptide

ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism

Cite this

Testes exhibit elevated expression of calcitonin gene-related peptide receptor component protein. / Balkan, Wayne E; Oates, Edward L.; Howard, Guy; Roos, Bernard A.

In: Endocrinology, Vol. 140, No. 3, 09.03.1999, p. 1459-1469.

Research output: Contribution to journalArticle

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