Test of some aging hypotheses using two-dimensional protein mapping

D. L. Wilson, M. E. Hall, G. C. Stone

Research output: Contribution to journalArticle

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Abstract

The authors have compared the proteins from superior cervical sympathetic ganglia (SCSG) in young and old male Fischer rats. They have searched for the kinds of changes in protein size or charge which are predicted by such cellular aging theories as the error catastrophe theory, the somatic mutation theory, the cross-linkage theory, translational aging, the deamidation theory, and the autoimmune theory. SCSG from young (2 months to 1 year) and old (21-24 months) rats were labeled in vitro for 1 h with 14C-leucine prior to protein extraction for analysis. Protein analysis was on high-resolution, two-dimensional polyacrylamide gels using O'Farrell's technique, with some modifications. Proteins were separated according to isoelectric point in one dimension and molecular weight in the second dimension. The changes predicted by the above aging theories were detected neither in the staining pattern, showing protein amounts, nor in the labeling pattern on autoradiographs of dried gels, showing newly synthesized proteins. The results suggest the possibility that, at least for rat, some other mechanisms for aging are present.

Original languageEnglish
Pages (from-to)426-433
Number of pages8
JournalGerontology
Volume24
Issue number6
StatePublished - Dec 1 1978

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Proteins
Superior Cervical Ganglion
Sympathetic Ganglia
Cell Aging
Inbred F344 Rats
Isoelectric Point
Leucine
Molecular Weight
Gels
Staining and Labeling
Mutation
polyacrylamide gels
In Vitro Techniques

ASJC Scopus subject areas

  • Aging

Cite this

Wilson, D. L., Hall, M. E., & Stone, G. C. (1978). Test of some aging hypotheses using two-dimensional protein mapping. Gerontology, 24(6), 426-433.

Test of some aging hypotheses using two-dimensional protein mapping. / Wilson, D. L.; Hall, M. E.; Stone, G. C.

In: Gerontology, Vol. 24, No. 6, 01.12.1978, p. 426-433.

Research output: Contribution to journalArticle

Wilson, DL, Hall, ME & Stone, GC 1978, 'Test of some aging hypotheses using two-dimensional protein mapping', Gerontology, vol. 24, no. 6, pp. 426-433.
Wilson DL, Hall ME, Stone GC. Test of some aging hypotheses using two-dimensional protein mapping. Gerontology. 1978 Dec 1;24(6):426-433.
Wilson, D. L. ; Hall, M. E. ; Stone, G. C. / Test of some aging hypotheses using two-dimensional protein mapping. In: Gerontology. 1978 ; Vol. 24, No. 6. pp. 426-433.
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