Test of some aging hypotheses using two-dimensional protein mapping

David L. Wilson, Michael E. Hall, George C. Stone

Research output: Contribution to journalArticle

20 Scopus citations

Abstract

The authors have compared the proteins from superior cervical sympathetic ganglia (SCSG) in young and old male Fischer rats. They have searched for the kinds of changes in protein size or charge which are predicted by such cellular aging theories as the error catastrophe theory, the somatic mutation theory, the cross-linkage theory, translational aging, the deamidation theory, and the autoimmune theory. SCSG from young (2 months to 1 year) and old (21-24 months) rats were labeled in vitro for 1 h with 14C-leucine prior to protein extraction for analysis. Protein analysis was on high-resolution, two-dimensional polyacrylamide gels using O'Farrell's technique, with some modifications. Proteins were separated according to isoelectric point in one dimension and molecular weight in the second dimension. The changes predicted by the above aging theories were detected neither in the staining pattern, showing protein amounts, nor in the labeling pattern on autoradiographs of dried gels, showing newly synthesized proteins. The results suggest the possibility that, at least for rat, some other mechanisms for aging are present.

Original languageEnglish (US)
Pages (from-to)426-433
Number of pages8
JournalGerontology
Volume24
Issue number6
DOIs
StatePublished - Jan 1 1978

Keywords

  • Cross-linkage
  • Error catastrophe
  • Nervous system
  • Polyacrylamide gels
  • Proteins
  • Rats
  • Sympathetic ganglion

ASJC Scopus subject areas

  • Aging
  • Geriatrics and Gerontology

Fingerprint Dive into the research topics of 'Test of some aging hypotheses using two-dimensional protein mapping'. Together they form a unique fingerprint.

  • Cite this