Tescalcin, a novel gene encoding a putative EF-hand Ca2+-binding protein, Col9a3, and Renin are expressed in the mouse testis during the early stages of gonadal differentiation

E. M. Perera, H. Martin, T. Seeherunvong, L. Kos, I. A. Hughes, J. R. Hawkins, G. D. Berkovitz

Research output: Contribution to journalArticlepeer-review

58 Scopus citations

Abstract

To identify genes that are differentially expressed in the developing testis we used representational difference analysis of complementary DNA from gonads of mouse embryos at 13.5 days postcoitum (dpc). Three genes were identified. One of them was a novel gene termed tescalcin that encoded a putative EF-hand Ca2+-binding protein. The open reading frame consisted of 642 nucleotides encoding a protein with 214 amino acids. Analysis of the predicted amino acid sequence revealed an N-myristoylation motif and several phosphorylation sites in addition to an EF-hand Ca2+-binding domain. Tescalcin messenger RNA (mRNA) was present in fetal testis, but not in ovary or mesonephros, and was restricted to the testicular cords. Its expression was first detected in the male gonad at 11.5 dpc and demonstrated a pattern consistent with a role in the testis at the early stages of testis differentiation. Tescalcin is expressed in the testis of KitW/W-v mice, indicating that it is not dependent on the presence of germ cells. The other two genes identified were collagen IX α3 (Col9a3) and Renin. Col9a3 expression was present at low levels in male and female gonads at 11.5 dpc. Thereafter, it was markedly up-regulated in the male, but remained very low in the female. Expression of Col9a3 was restricted to testicular cords and was also detected in testis of KitW/W-v mice. Renin mRNA was first detected in testis at 12.5 dpc, increased thereafter, and reached a peak at 16.5 dpc. Renin mRNA was localized in cells of the interstitium and cells at the border between the gonad and mesonephros. Expression of Renin in the ovary was not detected using standard conditions.

Original languageEnglish (US)
Pages (from-to)455-463
Number of pages9
JournalEndocrinology
Volume142
Issue number1
DOIs
StatePublished - 2001

ASJC Scopus subject areas

  • Endocrinology

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