Abstract
Objective. To evaluate the effect of beta-adrenergic agonists on the regulation of the expression of the placental corticotropin-releasing hormone (CRH) gene. Study design. Term placentae were collected at the time of elective cesarean section, and trophoblast cells were harvested, isolated, and cultured. The isolated trophoblasts were plated, cultured and subsequently treated with cortisol, terbutaline, RU486, or vehicle control. CRH expression, mRNA abundance of CRH, and the housekeeping gene beta-actin were evaluated by Northern blot analysis. Results. Exposure of the trophoblasts to terbutaline (10-8 M) inhibited the expression of the CRH gene as depicted by Northern blot analysis. Co-addition of terbutaline (10-8 M) and RU486 (10 -6 M) did not block the stimulatory effects of RU486 on placental trophoblast cells. Conclusion. The beta-adrenergic agonist terbutaline inhibits the expression of CRH in human trophoblasts. This finding may provide insight into the mechanism of action of terbutaline as a tocolytic agent.
| Original language | English |
|---|---|
| Pages (from-to) | 735-739 |
| Number of pages | 5 |
| Journal | Journal of Maternal-Fetal and Neonatal Medicine |
| Volume | 19 |
| Issue number | 11 |
| DOIs | |
| State | Published - Nov 1 2006 |
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Keywords
- Beta-adrenergic agonists
- CRH
- Placenta
- Preterm labor
- Progesterone
- Terbutaline
ASJC Scopus subject areas
- Pediatrics, Perinatology, and Child Health
- Obstetrics and Gynecology
Cite this
Terbutaline inhibits corticotropin-releasing hormone (CRH) expression in human trophoblast cells. / Kontopoulos, Eftichia V.; Chandras, Christina; Karalis, Katia.
In: Journal of Maternal-Fetal and Neonatal Medicine, Vol. 19, No. 11, 01.11.2006, p. 735-739.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Terbutaline inhibits corticotropin-releasing hormone (CRH) expression in human trophoblast cells
AU - Kontopoulos, Eftichia V.
AU - Chandras, Christina
AU - Karalis, Katia
PY - 2006/11/1
Y1 - 2006/11/1
N2 - Objective. To evaluate the effect of beta-adrenergic agonists on the regulation of the expression of the placental corticotropin-releasing hormone (CRH) gene. Study design. Term placentae were collected at the time of elective cesarean section, and trophoblast cells were harvested, isolated, and cultured. The isolated trophoblasts were plated, cultured and subsequently treated with cortisol, terbutaline, RU486, or vehicle control. CRH expression, mRNA abundance of CRH, and the housekeeping gene beta-actin were evaluated by Northern blot analysis. Results. Exposure of the trophoblasts to terbutaline (10-8 M) inhibited the expression of the CRH gene as depicted by Northern blot analysis. Co-addition of terbutaline (10-8 M) and RU486 (10 -6 M) did not block the stimulatory effects of RU486 on placental trophoblast cells. Conclusion. The beta-adrenergic agonist terbutaline inhibits the expression of CRH in human trophoblasts. This finding may provide insight into the mechanism of action of terbutaline as a tocolytic agent.
AB - Objective. To evaluate the effect of beta-adrenergic agonists on the regulation of the expression of the placental corticotropin-releasing hormone (CRH) gene. Study design. Term placentae were collected at the time of elective cesarean section, and trophoblast cells were harvested, isolated, and cultured. The isolated trophoblasts were plated, cultured and subsequently treated with cortisol, terbutaline, RU486, or vehicle control. CRH expression, mRNA abundance of CRH, and the housekeeping gene beta-actin were evaluated by Northern blot analysis. Results. Exposure of the trophoblasts to terbutaline (10-8 M) inhibited the expression of the CRH gene as depicted by Northern blot analysis. Co-addition of terbutaline (10-8 M) and RU486 (10 -6 M) did not block the stimulatory effects of RU486 on placental trophoblast cells. Conclusion. The beta-adrenergic agonist terbutaline inhibits the expression of CRH in human trophoblasts. This finding may provide insight into the mechanism of action of terbutaline as a tocolytic agent.
KW - Beta-adrenergic agonists
KW - CRH
KW - Placenta
KW - Preterm labor
KW - Progesterone
KW - Terbutaline
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UR - http://www.scopus.com/inward/citedby.url?scp=33751573314&partnerID=8YFLogxK
U2 - 10.1080/14767050600886724
DO - 10.1080/14767050600886724
M3 - Article
C2 - 17127497
AN - SCOPUS:33751573314
VL - 19
SP - 735
EP - 739
JO - Journal of Maternal-Fetal and Neonatal Medicine
JF - Journal of Maternal-Fetal and Neonatal Medicine
SN - 1476-7058
IS - 11
ER -