Terbutaline inhibits corticotropin-releasing hormone (CRH) expression in human trophoblast cells

Eftichia V. Kontopoulos; Christina Chandras; Katia Karalis

doi:10.1080/14767050600886724

Terbutaline inhibits corticotropin-releasing hormone (CRH) expression in human trophoblast cells

Eftichia V. Kontopoulos, Christina Chandras, Katia Karalis

Obstetrics & Gynecology

Research output: Contribution to journal › Article

2 Scopus citations

Abstract

Objective. To evaluate the effect of beta-adrenergic agonists on the regulation of the expression of the placental corticotropin-releasing hormone (CRH) gene. Study design. Term placentae were collected at the time of elective cesarean section, and trophoblast cells were harvested, isolated, and cultured. The isolated trophoblasts were plated, cultured and subsequently treated with cortisol, terbutaline, RU486, or vehicle control. CRH expression, mRNA abundance of CRH, and the housekeeping gene beta-actin were evaluated by Northern blot analysis. Results. Exposure of the trophoblasts to terbutaline (10^-8 M) inhibited the expression of the CRH gene as depicted by Northern blot analysis. Co-addition of terbutaline (10^-8 M) and RU486 (10 ^-6 M) did not block the stimulatory effects of RU486 on placental trophoblast cells. Conclusion. The beta-adrenergic agonist terbutaline inhibits the expression of CRH in human trophoblasts. This finding may provide insight into the mechanism of action of terbutaline as a tocolytic agent.

Original language	English (US)
Pages (from-to)	735-739
Number of pages	5
Journal	Journal of Maternal-Fetal and Neonatal Medicine
Volume	19
Issue number	11
DOIs	https://doi.org/10.1080/14767050600886724
State	Published - Nov 1 2006

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Keywords

Beta-adrenergic agonists
CRH
Placenta
Preterm labor
Progesterone
Terbutaline

ASJC Scopus subject areas

Pediatrics, Perinatology, and Child Health
Obstetrics and Gynecology

Cite this

Kontopoulos, E. V., Chandras, C., & Karalis, K. (2006). Terbutaline inhibits corticotropin-releasing hormone (CRH) expression in human trophoblast cells. Journal of Maternal-Fetal and Neonatal Medicine, 19(11), 735-739. https://doi.org/10.1080/14767050600886724

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abstract = "Objective. To evaluate the effect of beta-adrenergic agonists on the regulation of the expression of the placental corticotropin-releasing hormone (CRH) gene. Study design. Term placentae were collected at the time of elective cesarean section, and trophoblast cells were harvested, isolated, and cultured. The isolated trophoblasts were plated, cultured and subsequently treated with cortisol, terbutaline, RU486, or vehicle control. CRH expression, mRNA abundance of CRH, and the housekeeping gene beta-actin were evaluated by Northern blot analysis. Results. Exposure of the trophoblasts to terbutaline (10-8 M) inhibited the expression of the CRH gene as depicted by Northern blot analysis. Co-addition of terbutaline (10-8 M) and RU486 (10 -6 M) did not block the stimulatory effects of RU486 on placental trophoblast cells. Conclusion. The beta-adrenergic agonist terbutaline inhibits the expression of CRH in human trophoblasts. This finding may provide insight into the mechanism of action of terbutaline as a tocolytic agent.",

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AU - Karalis, Katia

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N2 - Objective. To evaluate the effect of beta-adrenergic agonists on the regulation of the expression of the placental corticotropin-releasing hormone (CRH) gene. Study design. Term placentae were collected at the time of elective cesarean section, and trophoblast cells were harvested, isolated, and cultured. The isolated trophoblasts were plated, cultured and subsequently treated with cortisol, terbutaline, RU486, or vehicle control. CRH expression, mRNA abundance of CRH, and the housekeeping gene beta-actin were evaluated by Northern blot analysis. Results. Exposure of the trophoblasts to terbutaline (10-8 M) inhibited the expression of the CRH gene as depicted by Northern blot analysis. Co-addition of terbutaline (10-8 M) and RU486 (10 -6 M) did not block the stimulatory effects of RU486 on placental trophoblast cells. Conclusion. The beta-adrenergic agonist terbutaline inhibits the expression of CRH in human trophoblasts. This finding may provide insight into the mechanism of action of terbutaline as a tocolytic agent.

AB - Objective. To evaluate the effect of beta-adrenergic agonists on the regulation of the expression of the placental corticotropin-releasing hormone (CRH) gene. Study design. Term placentae were collected at the time of elective cesarean section, and trophoblast cells were harvested, isolated, and cultured. The isolated trophoblasts were plated, cultured and subsequently treated with cortisol, terbutaline, RU486, or vehicle control. CRH expression, mRNA abundance of CRH, and the housekeeping gene beta-actin were evaluated by Northern blot analysis. Results. Exposure of the trophoblasts to terbutaline (10-8 M) inhibited the expression of the CRH gene as depicted by Northern blot analysis. Co-addition of terbutaline (10-8 M) and RU486 (10 -6 M) did not block the stimulatory effects of RU486 on placental trophoblast cells. Conclusion. The beta-adrenergic agonist terbutaline inhibits the expression of CRH in human trophoblasts. This finding may provide insight into the mechanism of action of terbutaline as a tocolytic agent.

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Access to Document

10.1080/14767050600886724