Temporal single-cell regeneration studies: the greatest thing since sliced pancreas?

Juan Domínguez-Bendala, Mirza Muhammad Fahd Qadir, Ricardo Luis Pastori

Research output: Contribution to journalReview articlepeer-review

Abstract

The application of single-cell analytic techniques to the study of stem/progenitor cell niches supports the emerging view that pancreatic cell lineages are in a state of flux between differentiation stages. For all their value, however, such analyses merely offer a snapshot of the cellular palette of the tissue at any given time point. Conclusions about potential developmental/regeneration paths are solely based on bioinformatics inferences. In this context, the advent of new techniques for the long-term culture and lineage tracing of human pancreatic slices offers a virtual window into the native organ and presents the field with a unique opportunity to serially resolve pancreatic regeneration dynamics at the single-cell level.

Original languageEnglish (US)
Pages (from-to)433-443
Number of pages11
JournalTrends in Endocrinology and Metabolism
Volume32
Issue number7
DOIs
StatePublished - Jul 2021

Keywords

  • human pancreatic slices
  • pancreatic regeneration
  • pseudotime
  • single-cell RNA sequencing
  • β-cell neogenesis

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Endocrinology

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