Temporal appearance of the presynaptic cytomatrix protein Bassoon during synaptogenesis

Rong G Zhai, Gisela Olias, Wook Joon Chung, Robin A J Lester, Susanne Tom Dieck, Kristina Langnaese, Michael R. Kreutz, Stefan Kindler, Eckart D. Gundelfinger, Craig C. Garner

Research output: Contribution to journalArticle

48 Citations (Scopus)

Abstract

Bassoon is a 420-kDa presynaptic cytomatrix protein potentially involved in the structural organization of neurotransmitter release sites. In this study, we have investigated a possible role for Bassoon in synaptogenesis and in defining synaptic vesicle recycling sites. We find that it is expressed at early stages of neuronal differentiation in which it is selectively sorted into axons. As synaptogenesis begins, Bassoon clusters appear along dendritic profiles simultaneously with synaptotagmin I, sites of synaptic vesicle recycling, and the acquisition of functional excitatory and inhibitory synapses. A role for Bassoon in the assembly of excitatory and inhibitory synapses is supported by the colocalization of Bassoon clusters with clusters of GKAP and AMPA receptors as well as GABA(A) receptors. These data indicate that the recruitment of Bassoon is an early step in the formation of synaptic junctions.

Original languageEnglish
Pages (from-to)417-428
Number of pages12
JournalMolecular and Cellular Neurosciences
Volume15
Issue number5
DOIs
StatePublished - May 1 2000
Externally publishedYes

Fingerprint

Synaptic Vesicles
Synapses
Synaptotagmin I
AMPA Receptors
GABA-A Receptors
Neurotransmitter Agents
Axons
Proteins

ASJC Scopus subject areas

  • Molecular Biology
  • Cellular and Molecular Neuroscience
  • Developmental Neuroscience

Cite this

Zhai, R. G., Olias, G., Chung, W. J., Lester, R. A. J., Tom Dieck, S., Langnaese, K., ... Garner, C. C. (2000). Temporal appearance of the presynaptic cytomatrix protein Bassoon during synaptogenesis. Molecular and Cellular Neurosciences, 15(5), 417-428. https://doi.org/10.1006/mcne.2000.0839

Temporal appearance of the presynaptic cytomatrix protein Bassoon during synaptogenesis. / Zhai, Rong G; Olias, Gisela; Chung, Wook Joon; Lester, Robin A J; Tom Dieck, Susanne; Langnaese, Kristina; Kreutz, Michael R.; Kindler, Stefan; Gundelfinger, Eckart D.; Garner, Craig C.

In: Molecular and Cellular Neurosciences, Vol. 15, No. 5, 01.05.2000, p. 417-428.

Research output: Contribution to journalArticle

Zhai, RG, Olias, G, Chung, WJ, Lester, RAJ, Tom Dieck, S, Langnaese, K, Kreutz, MR, Kindler, S, Gundelfinger, ED & Garner, CC 2000, 'Temporal appearance of the presynaptic cytomatrix protein Bassoon during synaptogenesis', Molecular and Cellular Neurosciences, vol. 15, no. 5, pp. 417-428. https://doi.org/10.1006/mcne.2000.0839
Zhai, Rong G ; Olias, Gisela ; Chung, Wook Joon ; Lester, Robin A J ; Tom Dieck, Susanne ; Langnaese, Kristina ; Kreutz, Michael R. ; Kindler, Stefan ; Gundelfinger, Eckart D. ; Garner, Craig C. / Temporal appearance of the presynaptic cytomatrix protein Bassoon during synaptogenesis. In: Molecular and Cellular Neurosciences. 2000 ; Vol. 15, No. 5. pp. 417-428.
@article{04231c9df5264d7a92f6c42764fbb0a1,
title = "Temporal appearance of the presynaptic cytomatrix protein Bassoon during synaptogenesis",
abstract = "Bassoon is a 420-kDa presynaptic cytomatrix protein potentially involved in the structural organization of neurotransmitter release sites. In this study, we have investigated a possible role for Bassoon in synaptogenesis and in defining synaptic vesicle recycling sites. We find that it is expressed at early stages of neuronal differentiation in which it is selectively sorted into axons. As synaptogenesis begins, Bassoon clusters appear along dendritic profiles simultaneously with synaptotagmin I, sites of synaptic vesicle recycling, and the acquisition of functional excitatory and inhibitory synapses. A role for Bassoon in the assembly of excitatory and inhibitory synapses is supported by the colocalization of Bassoon clusters with clusters of GKAP and AMPA receptors as well as GABA(A) receptors. These data indicate that the recruitment of Bassoon is an early step in the formation of synaptic junctions.",
author = "Zhai, {Rong G} and Gisela Olias and Chung, {Wook Joon} and Lester, {Robin A J} and {Tom Dieck}, Susanne and Kristina Langnaese and Kreutz, {Michael R.} and Stefan Kindler and Gundelfinger, {Eckart D.} and Garner, {Craig C.}",
year = "2000",
month = "5",
day = "1",
doi = "10.1006/mcne.2000.0839",
language = "English",
volume = "15",
pages = "417--428",
journal = "Molecular and Cellular Neuroscience",
issn = "1044-7431",
publisher = "Academic Press Inc.",
number = "5",

}

TY - JOUR

T1 - Temporal appearance of the presynaptic cytomatrix protein Bassoon during synaptogenesis

AU - Zhai, Rong G

AU - Olias, Gisela

AU - Chung, Wook Joon

AU - Lester, Robin A J

AU - Tom Dieck, Susanne

AU - Langnaese, Kristina

AU - Kreutz, Michael R.

AU - Kindler, Stefan

AU - Gundelfinger, Eckart D.

AU - Garner, Craig C.

PY - 2000/5/1

Y1 - 2000/5/1

N2 - Bassoon is a 420-kDa presynaptic cytomatrix protein potentially involved in the structural organization of neurotransmitter release sites. In this study, we have investigated a possible role for Bassoon in synaptogenesis and in defining synaptic vesicle recycling sites. We find that it is expressed at early stages of neuronal differentiation in which it is selectively sorted into axons. As synaptogenesis begins, Bassoon clusters appear along dendritic profiles simultaneously with synaptotagmin I, sites of synaptic vesicle recycling, and the acquisition of functional excitatory and inhibitory synapses. A role for Bassoon in the assembly of excitatory and inhibitory synapses is supported by the colocalization of Bassoon clusters with clusters of GKAP and AMPA receptors as well as GABA(A) receptors. These data indicate that the recruitment of Bassoon is an early step in the formation of synaptic junctions.

AB - Bassoon is a 420-kDa presynaptic cytomatrix protein potentially involved in the structural organization of neurotransmitter release sites. In this study, we have investigated a possible role for Bassoon in synaptogenesis and in defining synaptic vesicle recycling sites. We find that it is expressed at early stages of neuronal differentiation in which it is selectively sorted into axons. As synaptogenesis begins, Bassoon clusters appear along dendritic profiles simultaneously with synaptotagmin I, sites of synaptic vesicle recycling, and the acquisition of functional excitatory and inhibitory synapses. A role for Bassoon in the assembly of excitatory and inhibitory synapses is supported by the colocalization of Bassoon clusters with clusters of GKAP and AMPA receptors as well as GABA(A) receptors. These data indicate that the recruitment of Bassoon is an early step in the formation of synaptic junctions.

UR - http://www.scopus.com/inward/record.url?scp=0034123566&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0034123566&partnerID=8YFLogxK

U2 - 10.1006/mcne.2000.0839

DO - 10.1006/mcne.2000.0839

M3 - Article

VL - 15

SP - 417

EP - 428

JO - Molecular and Cellular Neuroscience

JF - Molecular and Cellular Neuroscience

SN - 1044-7431

IS - 5

ER -