Abstract
Lobsters (Homarus americanus) in the wild inhabit ocean waters where temperature can vary over a broad range (0-25°C). To examine how environmental thermal variability might affect lobster physiology, we examine the effects of temperature and thermal change on the acid-base status of the lobster hemolymph. Total CO2, pH, PCO2 and HCO 3- were measured in hemolymph sampled from lobsters acclimated to temperature in the laboratory as well as from lobsters acclimated to seasonal temperatures in the wild. Our results demonstrate that the change in hemolymph pH as a function of temperature follows the rule of constant relative alkalinity in lobsters acclimated to temperature over a period of weeks. However, thermal change can alter lobster acid-base status over a time course of minutes. Acute increases in temperature trigger a respiratory compensated metabolic acidosis of the hemolymph. Both the strength and frequency of the lobster heartbeat in vitro are modulated by changes in pH within the physiological range measured in vivo. These observations suggest that changes in acid-base status triggered by thermal variations in the environment might modulate lobster cardiac performance in vivo.
| Original language | English |
|---|---|
| Pages (from-to) | 1245-1254 |
| Number of pages | 10 |
| Journal | Journal of Experimental Biology |
| Volume | 210 |
| Issue number | 7 |
| DOIs | |
| State | Published - Apr 1 2007 |
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Keywords
- Acid-base balance
- Hemolymph
- Lobster
- pH
- Temperature
ASJC Scopus subject areas
- Agricultural and Biological Sciences(all)
- Agricultural and Biological Sciences (miscellaneous)
Cite this
Temperature and acid-base balance in the American lobster Homarus americanus. / Qadri, Syed Aman; Camacho, Joseph; Wang, Hongkun; Taylor, Josi R.; Grosell, Martin; Worden, Mary Kate.
In: Journal of Experimental Biology, Vol. 210, No. 7, 01.04.2007, p. 1245-1254.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Temperature and acid-base balance in the American lobster Homarus americanus
AU - Qadri, Syed Aman
AU - Camacho, Joseph
AU - Wang, Hongkun
AU - Taylor, Josi R.
AU - Grosell, Martin
AU - Worden, Mary Kate
PY - 2007/4/1
Y1 - 2007/4/1
N2 - Lobsters (Homarus americanus) in the wild inhabit ocean waters where temperature can vary over a broad range (0-25°C). To examine how environmental thermal variability might affect lobster physiology, we examine the effects of temperature and thermal change on the acid-base status of the lobster hemolymph. Total CO2, pH, PCO2 and HCO 3- were measured in hemolymph sampled from lobsters acclimated to temperature in the laboratory as well as from lobsters acclimated to seasonal temperatures in the wild. Our results demonstrate that the change in hemolymph pH as a function of temperature follows the rule of constant relative alkalinity in lobsters acclimated to temperature over a period of weeks. However, thermal change can alter lobster acid-base status over a time course of minutes. Acute increases in temperature trigger a respiratory compensated metabolic acidosis of the hemolymph. Both the strength and frequency of the lobster heartbeat in vitro are modulated by changes in pH within the physiological range measured in vivo. These observations suggest that changes in acid-base status triggered by thermal variations in the environment might modulate lobster cardiac performance in vivo.
AB - Lobsters (Homarus americanus) in the wild inhabit ocean waters where temperature can vary over a broad range (0-25°C). To examine how environmental thermal variability might affect lobster physiology, we examine the effects of temperature and thermal change on the acid-base status of the lobster hemolymph. Total CO2, pH, PCO2 and HCO 3- were measured in hemolymph sampled from lobsters acclimated to temperature in the laboratory as well as from lobsters acclimated to seasonal temperatures in the wild. Our results demonstrate that the change in hemolymph pH as a function of temperature follows the rule of constant relative alkalinity in lobsters acclimated to temperature over a period of weeks. However, thermal change can alter lobster acid-base status over a time course of minutes. Acute increases in temperature trigger a respiratory compensated metabolic acidosis of the hemolymph. Both the strength and frequency of the lobster heartbeat in vitro are modulated by changes in pH within the physiological range measured in vivo. These observations suggest that changes in acid-base status triggered by thermal variations in the environment might modulate lobster cardiac performance in vivo.
KW - Acid-base balance
KW - Hemolymph
KW - Lobster
KW - pH
KW - Temperature
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UR - http://www.scopus.com/inward/citedby.url?scp=34248384293&partnerID=8YFLogxK
U2 - 10.1242/jeb.02709
DO - 10.1242/jeb.02709
M3 - Article
C2 - 17371923
AN - SCOPUS:34248384293
VL - 210
SP - 1245
EP - 1254
JO - Journal of Experimental Biology
JF - Journal of Experimental Biology
SN - 0022-0949
IS - 7
ER -