Telomerase-transduced osteoarthritic fibroblast-like synoviocyte cell line

Yubo Sun, Gary S. Firestein, Leonor Wenger, Chun-Yuh Huang, Herman S Cheung

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

To examine whether the life span of fibroblast-like synoviocytes (FLSs) can be extended and to establish FLS cell lines that preserve the characteristics of primary FLSs, we introduced human catalytic subunit of telomerase (hTERT) gene into human osteoarthritic (OA) FLSs. Two hTERT-transduced clonal cell lines were established and one line, hTERT-OA FLS 13A, was characterized. The hTERT-OA FLS 13A cells have a morphology similar to that of the parental untransduced cells and a population-doubling time similar to that of the parental cells of early passages. While the parental untransduced OA FLSs reached senescence after 100 days in culture, the hTERT-OA FLS 13A cells continued to grow at a population-doubling rate of once in about every 2-3 days. The hTERT-OA 13A cells have so far grown in culture beyond 450 days and maintained the same growth rate. Furthermore, the hTERT-OA FLS 13A cells preserved their sensitivity and response to the treatment with basic calcium phosphate crystals and interleukin-1β. In conclusion, exogenous expression of telomerase represents a way to extend the life span of human FLSs and telomerase-transduced FLS cells offer a promising tool for gene regulation, cell-based assay, cell transplantation-based gene therapy, and tissue engineering research and development.

Original languageEnglish
Pages (from-to)1287-1292
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume323
Issue number4
DOIs
StatePublished - Oct 29 2004

Fingerprint

Telomerase
Fibroblasts
Cells
Cell Line
Synoviocytes
Transplantation (surgical)
Gene therapy
Engineering research
Cell Transplantation
Tissue Engineering
Interleukin-1
Tissue engineering
Cell culture
Gene expression
Genetic Therapy
Population
Genes
Assays

Keywords

  • BCP crystals
  • Cox-2
  • IL-1β
  • Life span
  • MMP-1
  • Osteoarthritis
  • Synoviocytes
  • Telomerase

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Telomerase-transduced osteoarthritic fibroblast-like synoviocyte cell line. / Sun, Yubo; Firestein, Gary S.; Wenger, Leonor; Huang, Chun-Yuh; Cheung, Herman S.

In: Biochemical and Biophysical Research Communications, Vol. 323, No. 4, 29.10.2004, p. 1287-1292.

Research output: Contribution to journalArticle

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