Technical report: SYBR Green I and the improved sensitivity of the single-cell electrophoresis assay

T. H. Ward, B. Marples

Research output: Contribution to journalArticle

22 Scopus citations

Abstract

The single-cell electrophoresis (comet) assay is an established method for measuring radiation-induced strand breaks in DNA. The extent of damage is quantified in individual cells by assessing the intensity and distribution of a fluorescent DNA-binding dye using image analysis software. Using the Kinetic Imaging Komet3 system, it is demonstrated that the fluorochrome SYBR Green I improves the resolution and sensitivity of the comet assay, particularly for measuring radiation-induced double strand breaks.

Original languageEnglish (US)
Pages (from-to)61-65
Number of pages5
JournalInternational Journal of Radiation Biology
Volume76
Issue number1
DOIs
StatePublished - Jan 1 2000
Externally publishedYes

ASJC Scopus subject areas

  • Radiological and Ultrasound Technology
  • Radiology Nuclear Medicine and imaging

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