Abstract
The single-cell electrophoresis (comet) assay is an established method for measuring radiation-induced strand breaks in DNA. The extent of damage is quantified in individual cells by assessing the intensity and distribution of a fluorescent DNA-binding dye using image analysis software. Using the Kinetic Imaging Komet3 system, it is demonstrated that the fluorochrome SYBR Green I improves the resolution and sensitivity of the comet assay, particularly for measuring radiation-induced double strand breaks.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 61-65 |
| Number of pages | 5 |
| Journal | International Journal of Radiation Biology |
| Volume | 76 |
| Issue number | 1 |
| DOIs | |
| State | Published - Jan 1 2000 |
| Externally published | Yes |
ASJC Scopus subject areas
- Radiological and Ultrasound Technology
- Radiology Nuclear Medicine and imaging