Abstract
The single-cell electrophoresis (comet) assay is an established method for measuring radiation-induced strand breaks in DNA. The extent of damage is quantified in individual cells by assessing the intensity and distribution of a fluorescent DNA-binding dye using image analysis software. Using the Kinetic Imaging Komet3 system, it is demonstrated that the fluorochrome SYBR Green I improves the resolution and sensitivity of the comet assay, particularly for measuring radiation-induced double strand breaks.
Original language | English (US) |
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Pages (from-to) | 61-65 |
Number of pages | 5 |
Journal | International Journal of Radiation Biology |
Volume | 76 |
Issue number | 1 |
DOIs | |
State | Published - Jan 1 2000 |
Externally published | Yes |
ASJC Scopus subject areas
- Radiological and Ultrasound Technology
- Radiology Nuclear Medicine and imaging