TCRγ gene rearrangement analysis in skin samples and peripheral blood of mycosis fungoides patients

Lidija Kandolf Sekulović, Bojana Cikota, Milica Popović, Olivera Stojadinović, Olivera Tarabar, Jelena Bašanović, Dušan Škiljević, Sonja Vesić, Ljiljana Medenica, Miloš Pavlović, Zvonko Magić

Research output: Contribution to journalArticlepeer-review

13 Scopus citations

Abstract

Background: Diagnosing mycosis fungoides (MF) can be challenging in the early stage of the disease because histopathological features may simulate a variety of benign inflammatory skin diseases. Assessment of T-cell clonality was found to be useful in diagnosis and follow-up of patients. Objective: In this study, PCR-based TCRγ gene rearrangement analysis was performed in skin and peripheral blood samples of patients with MF treated at the two largest referral centers in Serbia, and the results obtained were correlated with clinical and follow-up data. Methods: Skin and peripheral blood samples were obtained with informed consent from 37 patients treated at the Department of Dermatology of the Military Medical Academy and the Medical Center of Serbia from 2001 to 2006. The median time of follow-up was 4 years. Multiplex PCR was used for TCRγ gene rearrangement analysis in skin and peripheral blood samples. Clonality results were correlated with the clinical data and disease course data. Results: Monoclonality was detected in skin samples of 30/37 patients (81%), in 2/5 patients with large-plaque parapsoriasis (LPP), in 28/32 (88%) patients with histologically proven MF, and in 1/16 (6%) patients with benign inflammatory dermatoses. A monoclonal pattern in both skin and peripheral blood was detected in 7/16 (44%) patients in the late stage of the disease, and in 1/7 (14%) patients in the early stage of the disease. A dominant clone was found in both skin and peripheral blood in 1/4 patients in remission, 2/5 with a stable disease, and 4/9 (44%) with disease progression. Conclusion: TCR-γ gene rearrangement analysis can be regarded as a useful adjunct to diagnosis of epidermotropic lymphoproliferative disorders. The presence of a dominant clone in both the skin and peripheral blood was more frequently detected in late stages and in patients with disease progression, confirming the usefulness of clonality detection by TCR-γ gene rearrangement analysis in follow-up of patients with primary cutaneous T-cell lymphomas.

Original languageEnglish (US)
Pages (from-to)149-155
Number of pages7
JournalActa Dermatovenerologica Alpina, Pannonica et Adriatica
Volume16
Issue number4
StatePublished - Dec 2007

Keywords

  • Diagnosis
  • Mycosis fungoides
  • T-cell clonality
  • TCRγ rearrangement

ASJC Scopus subject areas

  • Dermatology

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