Abstract
Taxol, a microtubule stabilizing drug, induces the formation of numerous microtubule asters in the cytoplasm of mit otic cells (De Brabander, M., G. Geuens, R. Nuydens, R. Willebrords, J. DeMey. 1981. Proc. Natl. Acad. Sci. USA. 78:5608-5612). The center of these asters share with spindle poles some characteristics such as the presence of centrosomal material and calmodulin. We have recently reproduced the assembly of taxol asters in a cell-free system (Buendia, B., C. Antony, F. Verde, M. Bornens, and E. Karsenti. 1990. J. Cell Sci. 97:259-271) using extracts of Xenopus eggs. In this paper, we show that taxol aster assembly requires phosphorylation, and that they do not grow from preformed centers, but rather by a reorganization of microtubules first crosslinked into bundles. This process seems to involve sliding of microtubules along each other and we show that cytoplasmic dynein is required for taxol aster assembly. This result provides a possible functional basis to the recent findings, that dynein is present in the spindle and enriched near spindle poles (Pfarr, C. M., M. Cove, P. M. Grissom, T. S. Hays, M. E. Porter, and J. R. McIntosh. 1990. Nature (Lond.). 345:263-265; Steuer, E. R., L. Wordeman, T. A. Schroer, and M. P. Sheetz. 1990. Nature (Lond.). 345:266-268).
| Original language | English |
|---|---|
| Pages (from-to) | 1177-1187 |
| Number of pages | 11 |
| Journal | Journal of Cell Biology |
| Volume | 112 |
| Issue number | 6 |
| State | Published - Mar 1 1991 |
| Externally published | Yes |
Fingerprint
ASJC Scopus subject areas
- Cell Biology
Cite this
Taxol-induced microtubule asters in mitotic extracts of Xenopus eggs : Requirement for phosphorylated factors and cytoplasmic dynein. / Verde, Fulvia; Berrez, Jean Marc; Antony, Claude; Karsenti, Eric.
In: Journal of Cell Biology, Vol. 112, No. 6, 01.03.1991, p. 1177-1187.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Taxol-induced microtubule asters in mitotic extracts of Xenopus eggs
T2 - Requirement for phosphorylated factors and cytoplasmic dynein
AU - Verde, Fulvia
AU - Berrez, Jean Marc
AU - Antony, Claude
AU - Karsenti, Eric
PY - 1991/3/1
Y1 - 1991/3/1
N2 - Taxol, a microtubule stabilizing drug, induces the formation of numerous microtubule asters in the cytoplasm of mit otic cells (De Brabander, M., G. Geuens, R. Nuydens, R. Willebrords, J. DeMey. 1981. Proc. Natl. Acad. Sci. USA. 78:5608-5612). The center of these asters share with spindle poles some characteristics such as the presence of centrosomal material and calmodulin. We have recently reproduced the assembly of taxol asters in a cell-free system (Buendia, B., C. Antony, F. Verde, M. Bornens, and E. Karsenti. 1990. J. Cell Sci. 97:259-271) using extracts of Xenopus eggs. In this paper, we show that taxol aster assembly requires phosphorylation, and that they do not grow from preformed centers, but rather by a reorganization of microtubules first crosslinked into bundles. This process seems to involve sliding of microtubules along each other and we show that cytoplasmic dynein is required for taxol aster assembly. This result provides a possible functional basis to the recent findings, that dynein is present in the spindle and enriched near spindle poles (Pfarr, C. M., M. Cove, P. M. Grissom, T. S. Hays, M. E. Porter, and J. R. McIntosh. 1990. Nature (Lond.). 345:263-265; Steuer, E. R., L. Wordeman, T. A. Schroer, and M. P. Sheetz. 1990. Nature (Lond.). 345:266-268).
AB - Taxol, a microtubule stabilizing drug, induces the formation of numerous microtubule asters in the cytoplasm of mit otic cells (De Brabander, M., G. Geuens, R. Nuydens, R. Willebrords, J. DeMey. 1981. Proc. Natl. Acad. Sci. USA. 78:5608-5612). The center of these asters share with spindle poles some characteristics such as the presence of centrosomal material and calmodulin. We have recently reproduced the assembly of taxol asters in a cell-free system (Buendia, B., C. Antony, F. Verde, M. Bornens, and E. Karsenti. 1990. J. Cell Sci. 97:259-271) using extracts of Xenopus eggs. In this paper, we show that taxol aster assembly requires phosphorylation, and that they do not grow from preformed centers, but rather by a reorganization of microtubules first crosslinked into bundles. This process seems to involve sliding of microtubules along each other and we show that cytoplasmic dynein is required for taxol aster assembly. This result provides a possible functional basis to the recent findings, that dynein is present in the spindle and enriched near spindle poles (Pfarr, C. M., M. Cove, P. M. Grissom, T. S. Hays, M. E. Porter, and J. R. McIntosh. 1990. Nature (Lond.). 345:263-265; Steuer, E. R., L. Wordeman, T. A. Schroer, and M. P. Sheetz. 1990. Nature (Lond.). 345:266-268).
UR - http://www.scopus.com/inward/record.url?scp=0026071835&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0026071835&partnerID=8YFLogxK
M3 - Article
C2 - 1671864
AN - SCOPUS:0026071835
VL - 112
SP - 1177
EP - 1187
JO - Journal of Cell Biology
JF - Journal of Cell Biology
SN - 0021-9525
IS - 6
ER -